2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38583Rhizoma Paridis is a perennial herbaceous plant of the Paris genus (belong to Liliaceae family). It is one of the traditional medical plants in China. Steroidal saponins (glycosides) existed in Rhizoma Paridis are the most important bioactive compounds, which have many biological activities such as anti-cancer, anti-thrombotic, anti-viral, hemolytic, hypocholesterolemic and hypoglycemic. Lai et al. (2003) has explored the plants recently and found three Rhizoma Paridis cultivars (Paris formosana HAYATA, Paris arisanensis HAYATA and Paris lancifolia HAYATA) growing in Taiwan. There are, however, no thorough report concerning the steroidal saponins in the Taiwanese Rhizoma Paridis cultivars. Therefore, it is very import to know the components and contents of saponins in these Rhizoma Paridis plants for their exploitation. In the investigation, anti-cancer abilities of these purified Rhizoma Paridis saponins were also evaluated with many kinds of carcinona cell lines; moreover, the relationships between the abilities and saponin structures would be studied as well. The tentative schedule has been set as followings: First year: Rhizoma Paridis plants would be collected widely. TLC would be used to screen those containing steroidal saponins followed by isolation and identification of each saponin compound. The composition of steroidal saponins in Taiwanese Rhizoma Paridis plants would be confirmed in the stage. Second year: To develop an analytical protocol for routine works based on a high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and to determine the amount of individual saponin in Rhizoma Paridis cultivars. Third year: To evaluate anti-cancer abilities (with trypan blue dye exclusion assay, cell cycle assay, DNA fragmentation assay, determination of mitochondria membrane potential, etc) of the purified Rhizoma Paridis saponins using Hep G2 (Human hepatocellular carcinoma, ATCC No. HB-8065), HEK293 (human embryonic kidney epithelial cell line, ATCC No. CRL-1573), MCF7 (human breast carcinoma cell line, ATCC No. HTB-22), KB (human mouth epidermal carcinoma cell line, ATCC No.CCL-17), TSGH9201 (human gastric carcinoma cell line, BCRC No. 60146), MRC5 (human lung carcinoma cell line, ATCC No. CCL-171) cell lines and so on; moreover, the relationships between the abilities and saponin structures would also be studied further. The results will serve as a database for exploitation of Rhizoma Paridis curtivars growing in Taiwan.蚤休為為百合科（Liliaceae）重樓屬（Paris genus）多年生草本植物，其為中國傳統 的藥用植物。蚤休中所含的類固醇皂苷(steroidal saponins)為具有生理活性的重要化合 物。類固醇皂苷具有降血膽固醇、降血糖、減緩血小板凝集、抗病毒、抗癌等生理功效。 賴(2003) 近年來曾對台灣產蚤休植物進行調查，發現其有三個品種，分別為：台灣蚤休 （Paris formosana HAYATA）、阿里山蚤休（Paris arisanensis HAYATA）和狹葉蚤休 （Paris lancifolia HAYATA）。然而對於此些台灣產蚤休中接上醣基之類固醇皂苷研究 較少，對於其中的皂苷組成與含量仍然不甚清楚，有必要加以釐清，以利台灣產中草藥 之開發與利用。對於台灣蚤休皂苷之抗癌功效，在本研究中擬以多種癌細胞株進行評 估，並進ㄧ步探討此些功效是否因蚤休皂苷配醣體部分結構形式之不同及其接上醣基之 形式不同而有所差異。 因此本研究擬以三年的時間完成下列目標： 1. 第一年：廣泛收集台灣產蚤休，先以TLC 確認含有類固醇皂苷者，並自其中分離各 個類固醇皂苷，再進行結構鑑定，以確認台灣產蚤休中的類固醇皂苷組成。 2. 第二年：以高效能液相層析法配合蒸發光散射檢測系統(Evaporative light scattering detector, ELSD)，建立一套可以做為例行檢測蚤休類固醇皂苷的萃取與分析方法，並 檢測各台灣產蚤休間類固醇皂苷組成比率的差異。確立目前各台灣產蚤休中類固醇 皂苷的含量。 3. 第三年：採用Hep G2 (Human hepatocellular carcinoma, ATCC No. HB-8065)、HEK293 (human embryonic kidney epithelial cell line, ATCC No. CRL-1573)、MCF7 (human breast carcinoma cell line, ATCC No. HTB-22)、KB (human mouth epidermal carcinoma cell line, ATCC No.CCL-17)、TSGH9201(human gastric carcinoma cell line, BCRC No. 60146)和MRC5 (human lung carcinoma cell line, ATCC No. CCL-171)等細胞株，藉以 評估此些蚤休類固醇皂苷之抗癌活性(細胞存活率分析、細胞週期分析、DNA 片段 化分析及細胞內粒線體膜電位之分析等) ，並探討其活性與皂苷結構間之關係。 研究結果將歸納整理成一個資料庫，以提供台灣產蚤休之開發與利用。分析抗癌細胞實驗分離純化蚤休固醇類皂?AnalysisAnti-cancerCell experimentIsolationPurifyRhizoma ParidisSteroidal saponinProject