2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38581Both hyaluronic acid (HA) and collagen are major components of extracellular matrix (ECM) in the cartilage, they exerts effects on chondrocyte cell shape, phenotype and metabolism. In order to mimic the biological environment for chondrocytes growth, we will prepare the covalently binding HA-collagen fibers to promote the chondrogenic differentiation and proliferation of stem cell. In previously study, HA-collagen composite was synthesized by a 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated coupling reaction and the reaction mechanism impaired collagen fibrillogenesis. To reduce possible effect on collagen fibrillogenesis, HA will modified with 1,1'-carbonyldiimidazole (CDI), then the activated HA reacted with primary amino groups of collagen. Synthesize the copolymer in two steps, we can also change the feed ratio of HA to collagen. Furthermore, we will utilize the ATDC5 cell line, a well-characterized chondrogenic cell line derived from a mouse teratocarcinoma, and culture in HA-collagen fibers. The biological role of HA-Collagen fibers in the chondrogenic potential of stem cells will be analyzed by examining the cell morphology、cell proliferation、gene expression and cell-material interaction.透明質酸（Hyaluronic acid，HA）和膠原蛋白（Collagen）是軟骨組織細胞外 基質的主要成分，其存在對軟骨細胞的形狀、型態和代謝有重要的意義。為製備 仿生性之細胞載體，本研究選擇以化學鍵結方式偶合透明質酸和膠原蛋白 （HA-Collagen）作為基材，引導軟骨細胞的增生和分化。先前的研究是利用 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)偶合透明質酸和膠原蛋白， 不過EDC 的作用會使得膠原蛋白失去重組為膠原纖維的能力。因此本研究中選 擇以1,1'-carbonyldiimidazole (CDI)為架橋偶合透明質酸和膠原蛋白。此方法不但 可以控制膠原蛋白上透明質酸的偶合量，也保留兩分子上的官能性基團，減少對 膠原蛋白重組的影響。為評估此複合材料對幹細胞軟骨化的影響，我們選擇來自 鼠科動物之畸胎瘤細胞ATDC5 作為細胞來源，和HA-Collagen 纖維共同培養進 行軟骨化誘導。HA-Collagen 基材對軟骨化過程的生物效應則是透過對軟骨化細 胞的型態、增生、基因表現以及細胞材料間的交互作用等研究證實。透明質酸膠原蛋白11'-carbonyldiimidazole(CDI)hyaluronic acidcollagen11'-carbonyldiimidazole(CDI)Project