2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37680A fungal immunomodulatory protein (FIP-gts) has been isolated and purified from the mycelium of Ganoderma tsugae. The apparent molecular mass of FIP-gts was determined by SDS/PAGE with 13 kDa. The immunomodulatory activity of FIP-gts was demonstrated by its stimulatory activity toward human peripheral blood lymphocytes and remarkable induction of interferon-gamma (IFN-r) cytokine through the induction of ICAM-1 (Intercellular adhesion molecule-1). It may play an role on stimulating helper T1 cell pathway. We have observed the high similarity between FIP-gts and another immunomodulatory protein, FIP-fve, isolated from Flammulina velutipes. FIP-fve have shown to suppress systemic anaphylaxis reactions and local swelling of mouse edema by compound 48/80. Ganoderma, an oriental fungus, has been widely used as a remedy for promotion of health and longevity. It was reported to be effective in the treatment of chronic hepatopathy and anti-tumor activity. But, the immunomodulatory activity of FIP-gts will be further clarified. In asthma patients, it is characterized by skewed polarization of helper T cell (Th) development toward T helper cells type 2 (Th2) with increased production of interleukin 4 (IL-4) and IL-5, which affect immunoglobulin (Ig) isotype switching toward IgE, and differentiation, growth of eosinophils, respectively. Immune responses to protein antigens are strongly influenced by the nature of the helper T lymphocyte (Th) subsets participating in the response. Once deviation toward a Th1 or Th2 response begins, there is potential for further polarization driven by the Th cells themselves. FIP-gts have been proved the immunomodulatory effect by I.P and In vitro study. The aim of the present study will to examine the in vivo effect of FIP-gts by oral test on a murine model of asthma induced with Dermatophagoides pteronyssinus derived DerpII allergen. BALB/C mice immunized with Derp II. We will claim the health effect of Ganoderma tsugae.松杉靈芝(Ganoderma tsugae)中菌絲體中分離得免疫調節蛋白質 (FIP-gts)，其分子量13 kDa。此蛋白質對於人類周邊血液細胞具有刺激增殖並誘發干擾素-r大量表現，並透過誘導活化ICAM-1，進而促進Th1路徑有關。又與其胺基酸序列有78 % 相同的金針菇(FIP-fve)在動物試驗中已被證實可當作免疫抑制劑；減緩由BSA所引起的全身性休克反應及明顯減小由發炎物 compound 48/80所誘發的水腫。今年我們並已將此基因選殖並表現其蛋白質，並已證實它已具有誘發interferon-r的功能。靈芝一直以來被視為健康食品的一員，已證實具有保護肝臟、抗腫瘤等功能。但其免疫調節功能尚未澄清。過敏性氣喘一直是大家關心的事，尤其是許多人從事改善過敏體質與減緩病症著手。已知Th1與Th2型的細胞激素會互相拮抗。台灣過敏性氣喘患者的主要空氣過敏原由塵璊所引起氣喘患者的Ig E非常高， IL-4也高及esoinophil較多，這型患者多偏向進行Th2路徑。靈芝免疫調節功能雖然我們已在Balb/C老鼠以腹腔注射試驗及in vitro試驗中皆已證實具有免疫調節功能。由於擬闡釋發展靈芝為具有免疫調節功能的保健食品，所以本研究計畫擬繼續探討先純化松杉靈芝蛋白(FIP-gts) ，以餵食老鼠的方式探討是否具有減緩受塵璊過敏原 Der PII將老鼠致敏成氣喘症老鼠的症狀，亦或可減輕氣喘老鼠的病徵。發展出預防獲改善由塵璊所引起的氣喘之保健食品。氣喘保健食品松杉靈芝免疫調節蛋白AsthemaProtective foodFIP-gtsProject