潘惠錦Hui-Chin Pan陳虹均HUNG-CHUN CHEN生物醫學科學學系2026-03-182021-09-2820212021-07-30https://handle.ncl.edu.tw/11296/d32z2khttps://scholars.csmu.edu.tw/handle/123456789/5969MBNL3 是一種調節選擇性剪接的 RNA 結合蛋白，主要在增殖組織中表達。據報導，在許多癌症中MBNL3扮演促進的角色，例如乳腺癌、肝癌、非小細胞肺癌等。先前，我們實驗室發現卵巢癌細胞株中的 MBNL3 表現量高於正常卵巢細胞，並且 MBNL3 弱化會降低卵巢癌細胞的生長速度。我們想確定 MBNL3的表達如何影響細胞。我培養卵巢癌HeyC2細胞，用si-MBNL3轉染細胞弱化MBNL3表現量，並以si-NC作為對照組。使用 RT-PCR 和西方點墨法檢查弱化MBNL3後，確認MBNL3 表達量降低了大約50%。我們通過傷口癒合、transwell 和聚落形成試驗觀察了細胞行為。另一方面，我也使用Hela細胞轉染DNA，過量表達 MBNL3，再用相同的方式觀察細胞行為。我們的結果表明，弱化 HeyC2 中的 MBNL3 降低了細胞的遷移能力，以及細胞聚落的形成；而過表達MBNL3可促進Hela細胞遷移及增殖的能力。我也探討在卵巢癌中MBNL3與長非編碼lnc-PXN-AS1的關係，利用qRT-PCR檢測，結果顯示MBNL3可能藉由調控lnc-PXN-AS1的剪切，影響下游黏著斑蛋白的表現，促進腫瘤細胞的遷移侵襲與生長。MBNL3 is an RNA-binding protein that regulates alternative splicing and is mainly expressed in proliferative tissues. It has been reported that MBNL3 plays a promotive role in many cancers, such as breast cancer, liver cancer, non-small lung cancer and other cancers. Previously, our laboratory discovered higher MBNL3 expression in ovarian cancer cell lines than in normal ovarian cells, and MBNL3 knockdown decreased the growth rate of ovarian cancer cells. To determine how MBNL3 expression impacts on the cells, we cultured ovarian cancer HeyC2 cells and transfected the cells with si-MBNL3 to knockdown MBNL3, as well as with si-NC as a control. We confirmed decreased MBNL3 expression after MBNL3 knockdown using RT-PCR and Western blotting. And we examined cell behaviors by wound healing, transwell, and colony formation assays. On the other hand, we transfected DNA to overexpress MBNL3 in Hela cells, and examined the cell behaviors in the same way. Our results revealed that knockdown MBNL3 in HeyC2 decreased the migration ability of the cells, as well as the colony formation ability. Overexpression of MBNL3 in Hela cell also promoted cell migration and proliferation. I also explored the relationship between MBNL3 and long non-coding lnc-PXN-AS1 in ovarian cancer. Using qRT-PCR, the results showed that MBNL3 may affect downstream PXN expression by regulating the alternative splicing event of lnc-PXN-AS1, and thereby promotes the migration, invasion and growth of tumor cells.MBNL3HeyC2Helalnc-PXN-AS1卵巢癌細胞遷移聚落形成ovarian cancercell migrationcolony formationthesis