SHIANG-SUO HUANG2026-10-162026-10-162007-01-01Pure ID: 15336673Pure UUID: f2a36b88-a9e1-46ea-b5d0-9a79cd0c392f10.1253/circj.71.423https://www.scopus.com/pages/publications/33947171433https://scholars.csmu.edu.tw/handle/123456789/28178Background: Ischemic-reperfusion (IR) injury is a multifactorial process that leads to tissue damage and rejection in composite tissue allotransplantation (CTA). Antioxidant or free radical scavenger may reduce IR injury, so the effects of resveratrol, a natural antioxidant, on amelioration of leukocyte-endothelial cell adhesive interaction and prevention of transplant rejection in CTA were investigated. Methods and Results: In a microcirculatory study, resveratrol significantly reduced the number of IR-induced leukocytes rolling, adhering, and transmigrating in the postcapillary venules of the cremaster muscle. In the CTA study using groin skin flap allotransplantation across the MHC barrier 8-11-week-old Brown Norway donors (RT1n) and 10-11-week-old Lewis recipients (RT1l) rats were randomized into 4 groups: isograft control, allograft control, and 2 groups that received different doses of resveratrol (0.1 or 0.5 mg/kg) for 7 days. Allograft control animals rejected their allograft between 5 and 7 days postoperatively, whereas resveratrol-treated recipients had a moderate survival prolongation compared with the allograft control group. Consistent with these observations, histology results also showed reduction of lymphocytic infiltration and necrosis in resveratrol-treated subjects. Conclusion: Resveratrol treatment prolonged groin skin flap allotransplant survival in the recipient and ameliorated the leukocyte-endothelial cell adhesive interactions that may lead to attenuated and delayed rejection in CTA.6enjournal article