2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38786According to previous studies, cerebrovascular disease, cardiovascular diseases, and diabetes are related to lipid metabolism. In the progress of these diseases, improper accumulation of lipid caused lipotoxicity and derived oxidized low density lipoprotein (oxLDL) would be the major cause to promote the development of complication. In our previous study, we found a huge of lipid accumulated in the retina of atherosclerotic rabbit. We also found the diabetic rat exhibited hyperlipidemia, severe cataract, and irregular arrangement in retina. Base of those, lowering lipotoxicity and the effect of oxLDL in eyes would be a good strategy to decelerate the ophthalmopathy induced by metabolic diseases. Besides, it would be the future direction to find food or nature product to ameliorate ophthalmopathy. In foreign literature, several natural products are reported to possess the effect on protecting eyes, such as bilberry and blueberry, but in Taiwan, there is not yet full study to report any natural product possessing the eye-protection effect. In previous studies, mulberry extracts were proved rich in anthocyanins to possess the effects in inhibiting lipid peroxidation, formation of oxLDL, and foam cell formation. In our preliminary data, we found the components existing in mulberry, protocatechuic acid and cyanidin can reduce significantly oxLDL induced adult retinal pigmented epithelium cell (ARPE-19) toxicity. In this study, we aim to use mulberry water extract (MWE), mulberry polyphenols extract (MPE), and mulberry anthocyanins extract (MAE) to study the effects in ophthalmopathy induced by metabolic diseases. In the first year, the streptozotocin induced diabetes model will be used to observe the effects of mulberry extract in diabetic eyes, accompanying by exploring the relationships between oxLDL and diabetic eye. In the second year, we will use high fat diet to induce obese hamster in order to examine the effects of mulberry in obesity related eye diseases and the relationships between oxLDL and obesity inducing eye diseases. In the third year, ARPE-19 cell will be used to clarify the possible mechanisms in mulberry extract decelerating oxLDL-induced ophthalmopathy. The results will provide scientific evidences to evaluate the possibility for developing mulberry extract to a functional food in eye-protection.依據先前的研究發現，腦血管疾病、心臟疾病、糖尿病接與脂質 代謝相關，在此類疾病併發症的發生過程中，脂質不當堆積所引起的 脂質毒性 (lipotoxicity)以及衍生出之oxidized low density lipoprotein (oxLDL)可能為引起併發症的重要原因。我們在動脈粥狀硬化的兔子 眼部發現有脂質大量堆積的現象，我們也發現糖尿病動物血脂肪甚高 且眼部有嚴重白內障及視網膜排列不規則的現象，因此，降低 lipotoxicity 及oxLDL 對眼部的影響，應可有效抑制代謝性疾病引起眼 病變的併發症。另外，由食物或天然植物中找尋有效的抑制lipotoxicity 及oxLDL 並延緩眼病變併發症的成分更為未來發展的方向。在國外 文獻中已有許多相關天然物被研究，如山桑子、藍莓等，但在國內則 尚未有完整研究指出本土天然物對眼睛保健之功效。 先前研究已分析出桑椹具有花青素等天然抗氧化成分，並在花青 素萃物的功效上證實其具有抑制脂質過氧化、oxLDL 及foam cell 形 成的能力。本研究室以桑葚中成分protocatechuic acid 及cyanidin 預 處理人類視網膜色素上皮細胞 (Adult retinal pigmented epithelium cell, ARPE-19) 後加入oxLDL，發現桑葚成分可有效降低 oxLDL 引起的 cell toxicity。 本研究擬使用桑椹水萃物(MWE)、桑椹多酚萃物(MPE)、桑椹花 青素萃物(MAE)，在第一年針對糖尿病引起的眼病變觀察其延緩的成 效，並檢查因糖尿病引起的oxLDL 與眼病變的相關性。第二年則針 對肥胖造成之脂質代謝異常引起的眼病變觀察桑椹萃物有無延緩效 果及其與oxLDL 之相關性及可能機轉。第三年則以細胞實驗探討 MWE、MPE、MAE 延緩oxLDL 引起視網膜色素上皮細胞凋謝死亡 的作用及可能機轉，藉著研究成果進一步評估發展桑葚萃物成為眼睛 保健食品之可能性。桑葚糖尿病肥胖氧化型低密度脂蛋白視網膜病變白內障老化相關性黃斑部病變MulberryDiabetesObesityoxidized low density lipoproteinretinopathycataractage-related macular degenerationProject