YI-CHYI LAI2026-10-162026-10-162003-01-21Pure ID: 14980228Pure UUID: 7995b7b8-6f0f-4837-85a0-099b7e1cd2d610.1016/S0378-1097(02)01120-5https://www.scopus.com/pages/publications/0037458144https://scholars.csmu.edu.tw/handle/123456789/30092A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.6enjournal article