2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38580Over the pass decade, advances in understanding carcinogenesis have made possible the identification of the candidates of chemopreventive agents that are being developed to hit the key molecular targets. The most different between cancer cell and normal cell is that cancer cell can not control their growth rate and death. Nuciferine Leaf extract (NLE) has been identified a lot of benefit about anti-oxidant and reduced risks of cardiovascular disease. In this study aimed to define the anti-carcinogenesis and the mechanism of NLE reduced chemical-induced cancer and xenograft tumor in animal model. First we'll found (1) NLE extracted from Nuciferine Leaf will be separated and identified by HPLC; (2) animal model of chemical induced carcinogensis; (3) NLE suppresses the chemical induced incidence of mammary tumors in rats; (4) NLE suppresses tumor multiplicity of chemical induced mammary tumors; (5) NLE suppresses tumor volume of chemical induced mammary tumors; (6) mechanism of NLE suppresses chemical induced mammary tumor The results of these studies may be used to (1) develop a new chemopreventive agent.；(2) demonstrate the mechanisms of action of NLE in anticarcinogenesis.癌症預防已為世界趨勢，因此尋找特殊化學預防物質為現今之重要工作。有許多報告證實荷葉具抗氧化活性、降血脂等作用。此外，許多研究單位發現，癌細胞與正常細胞差別為無法進行一般細胞凋亡，因此生長無法控制。所以本研究擬以動物實驗模式深入探討台灣之荷葉萃取物抑制腫瘤生長之功效，並進一步探討其中抑制之機制。主要方向為(1)分離及萃取荷葉萃取物；(2)找尋適當之化學性誘導動物產生癌症之模式；(3)評估荷葉萃取物之抑制腫瘤的效果；(4)評估荷葉萃取物之抑制腫瘤的數目；(5) 評估荷葉萃取物之抑制腫瘤的大小；(6)探討其中之抑制機轉，找出荷葉萃取物之重要之target，如FAS (fatty acid synthase)，ER (estrogen receptor) 等。預期完成效益有(1) 分離及萃取荷葉萃取物；(2) 建立適當之化學性誘導動物產生癌症之模式；(3) 完成荷葉萃取物之抑制腫瘤的效果；(4) 完成荷葉萃取物之抑制腫瘤生長的速度；(5) 完成荷葉萃取物之抑制腫瘤的數目；(6) 完成荷葉萃取物之抑制腫瘤的大小；(7) 探討其中之抑制機轉，找出重要之target；(8) 推廣荷葉萃取物為降低癌症發生率之保健食品。從以上結果我們可以提出荷葉萃取物可以預防癌症之發生，並說明其中抑癌機轉。由於荷葉在國內易種植，因此本研究成果可應用於發展為降低癌症發生率之健康食品。Project