楊順發YANG, SHUN-FA鄭紓涵CHENG, SHU-HANSHU-HANCHENG醫學研究所碩士班2026-10-292026-07-3120262026-07-22https://handle.ncl.edu.tw/11296/k3um5zhttps://scholars.csmu.edu.tw/handle/123456789/36776口腔癌是臺灣常見癌症之一，且以男性為主，與吸菸、飲酒、口腔衛生不良及嚼食檳榔等危險因子相關。10,11-脫氫彎孢霉素　(10,11-Dehydrocurvularin，DCV) 為麴黴屬真菌次級代謝物，具有抗發炎及誘導細胞凋亡之活性。然而，DCV對口腔癌之作用機制仍尚未明確，因此本研究探討DCV是否具有抑制口腔癌細胞生長及誘導細胞凋亡之能力。細胞存活率試驗結果顯示，DCV可顯著抑制人類口腔癌細胞生長。Annexin V/PI染色分析亦證實DCV能有效促進細胞凋亡。進一步利用人類凋亡蛋白質陣列分析發現，DCV可提升cleaved caspase-3並降低cIAP-1及XIAP之表現量。Western blot分析結果顯示，隨DCV處理濃度增加，cleaved caspase-3/8/9之蛋白表現明顯上升，進一步證實DCV可透過活化caspase相關路徑誘導細胞凋亡。此外，DCV亦可活化MAPK訊息傳遞路徑。另外，細胞週期分析顯示，DCV處理後細胞出現G2/M期停滯，Western blot結果顯示G2/M期相關蛋白Cdc25c表現下降，推測DCV誘導之G2/M期細胞週期停滯可能與Cdc25c蛋白調控有關。綜合上述結果，DCV可誘導細胞凋亡及細胞週期G2/M期停滯，抑制人類口腔癌細胞生長。未來將進一步探討其分子作用機制，作為口腔癌治療之潛在應用依據。Oral cancer is a common malignancy in Taiwan, with major risk factors including smoking, alcohol consumption, poor oral hygiene, and betel quid chewing. 10,11-Dehydrocurvularin (DCV), a secondary metabolite from Aspergillus species, has anti-inflammatory and pro-apoptotic activities; however, its effects on oral cancer remain unclear. This study investigated the effects of DCV on human oral cancer cells. Analysis of cell viability showed that DCV significantly inhibited cell proliferation, and Annexin V/PI staining confirmed apoptosis induction. Protein analysis revealed upregulation of cleaved caspase-3 and downregulation of cIAP-1 and XIAP. Western blot further demonstrated increased cleaved caspase-3/8/9 expression in a dose-dependent manner, indicating activation of the caspase cascade. DCV also activated the MAPK pathway and induced G2/M phase arrest, accompanied by decreased Cdc25c expression. In conclusion, DCV suppresses oral cancer cell growth by inducing apoptosis and G2/M arrest, suggesting its potential as an anticancer agent.口腔癌1011-脫氫彎孢霉素細胞凋亡Oral cancer11-Dehydrocurvularinapoptosisthesis