蔡蕙芳Hui-Fang Tsai蔡沛珊Pei-Shan Tsai醫學檢驗暨生物技術學系2026-10-169999-12-3120212021-07-16https://handle.ncl.edu.tw/11296/dqp63dhttps://scholars.csmu.edu.tw/handle/123456789/22180第三型小腦脊髓運動失調症(Spinocerebellar ataxia type 3, SCA3)是多麩胺酸突變的顯性神經退化性遺傳疾病，由ATXN3基因中CAG三核苷酸的異常擴增所造成，導致轉譯出突變蛋白質ataxin-3，而產生活性氧化物(Reactive oxygen species, ROS)造成氧化壓力上升導致細胞凋亡，目前並無有效治療SCA3的方法。咖啡酸苯乙脂(CAPE)是蜂膠中的黃酮類化合物，具有多種生物學特性如抗發炎和抗氧化等。在本實驗中，主要是探討CAPE對H2O2誘導的SK-N-SH-MJD78細胞的神經保護作用。利用MTT細胞存活率分析、Acridine Orange、Hoechst染色、流式細胞儀與西方墨點法檢測神經保護作用。結果顯示CAPE能增加暴露在H2O2的SK-N-SH-MJD78細胞存活率並減少細胞凋亡，而在細胞前處理CAPE作用後粒線體凋亡途徑相關蛋白量、熱休克蛋白量及自噬相關蛋白量表現增加。此外，CAPE也能抑制活性氧的產生，在H2O2誘導的抗氧化蛋白表現量下降，也同樣能透過CAPE處理後恢復。實驗結果顯示CAPE 在體外SCA3模型中對H2O2誘導的細胞具有保護潛力，並且可能是預防第三型小腦脊髓運動失調症的潛在治療藥物。Spinocerebellar ataxia type 3 (SCA3) is a common polyglutamine disease. It is caused by the abnormal amplification of CAG trinucleotide in the ATXN3 gene, producing the mutant ataxin-3 protein. Reactive oxygen species (ROS) which is produced by mutant cell leads to the rising of cell oxidative stress and apoptosis. There is no effective treatment for SCA3. Caffeic acid phenethyl ester (CAPE) is a flavonoid in propolis, which has a variety of biological activities such as anti-inflammatory and antioxidant. In this experiment, the neuroprotective effect of CAPE on SK-N-SH-MJD78 cells was studied. Acridine Orange, Hoechst staining and Western blot were used to detect cell viability. In addition, antioxidant activity was measured by flow cytometry and Western blot. The result showed that low concentration of CAPE can increase the survival rate of SK-N-SH-MJD78 cells which exposed to H2O2. The level of cell apoptosis protein was decreaced by adding H2O2, but they partially recovered after CAPE treatment. In addition, CAPE also inhibited the production of reactive oxygen species. The decreasing levels in antioxidant protein induced by H2O2 also restored after CAPE treatment. These results show the neuroprotective potential of CAPE against H2O2-induced apoptosis in SCA3 in vitro model. Thus, CAPE may be a potential therapeutic compound to prevent Spinocerebellar ataxia type 3.第三型小腦脊髓運動失調症氧化壓力咖啡酸苯乙脂Spinocerebellar ataxia type 3Oxidative stressCaffeic acid phenethyl esterthesis