2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37910Tumor prevention is the tide current of the world. Therefore, searching for the chemopreventive agent will be the most important work. Anthocyanins and polyphenoic acid widespread present in the vegetable and fruits. Many reporters demonstrate that anthocyanins and polyphenoic acid have anti-oxidant activity, decrease cholesterol, tumor prevention. In this study also found that anthocyanins which extract dried calyx of Hibiscus sabdariffa L. (HAs) have anti-oxidant and protect the function of liver (Wang et al, Fd. Chem. Toxicol. 35, 1159-1164, 1997; 38, 411-416, 2000). Recent data shown that HAs and HPA would promote several kinds of cells apoptosis, such as Human gastric carcinoma AGS, mouse NIH/Swiss embryo cells, hepatocellular carcinoma Hep 3B, colorectal adenocarcinoma Caco-2, hepatoblastoma HepG2, adenocarcinoma MCF-7, human oral epidermoid carcinoma KB and human promyelocytic leukemia HL-60 (Fig. 1-3 and Table 1-3). Hence, this study will further investigate that the tumor prevention of HAs and HPA and molecular mechanism. The first years work would explore the function of HSE which are water extract from Hibiscus sabdariffa L. and its functional compounds HAs, HPA. The items of research including: 1. analyze HSE, HAs, HPA to promote several kinds of cells apoptosis by flow cytometer and DNA electrophoresis; 2. examine apoptosis-related gene expression, for example bcl-2 family, caspases, cytochrome c; 3. detect HSE, HAs, HPA to effect the cell cycle of cancer cells, contain cyclins, CDKs and associate each other, Rb phosphorylation and with E2F association as well as cell cycle distribution. We expect HSE, HAs, HPA maybe could promote cancer cells apoptosis and suppress cell cycle progression. Base on the data, we can explain how HSE, HAs, HPA could prevent and treat cancer and molecular mechanisms. According to these results, we expect it could application for developing a new chemopreventive agent.癌症預防已為一世界潮流，因此，尋找一化學預防物質（chemopreventive agent）為一重要工作。花青素（Anthocyanins）及多酚酸（Polyphenoic acid）廣存於蔬菜、水果等植物中，已有許多報告證明花青素及多酚酸具抗氧化活性，降低血脂肪及防癌等作用，本研究室也發現分離自洛神花之花青素（Hibiscus anthocyanins, HAs）有抗氧化及保肝之功能（Wang et.al, Fd. Chem. Toxicol. 35, 1159-1164, 1997；38, 411-416, 2000）。最近的數據也顯示此類HAs及Hibiscus polyphenoic acid（HPA）會促進胃癌（AGS），肝癌（3B, G2），口腔上皮癌（KB），血癌（HL-60），乳癌（MCF-7），腸癌（Caco-2）等細胞凋謝死亡（如圖一、二、三及表一、二、三），因此，本研究擬深入探討HAs, HPA之抗癌作用及其作用之分子機制，並加以建立HAs及HPA之分析。 第一年的工作探討洛神花萃取物（Hibiscus Sabdaffra extracts, HSE）及其功能性成份HAs及HPA抗癌之作用，工作之項目包括（1）利用flow cytometer 及DNA electrophoresis 分析HSE, HAs, HPA促進各種癌細胞凋謝死亡；（2）探討apoptosis-related gene expression，包括bcl-2 family, caspase, cyt c；（3）探討HSE, HAs, HPA對癌細胞cell cycle的影響，包括各種Cyclins及CDKs及它們的association, Rb phosphorylation及與E2F之association, cell cycle分佈等，由以上的結果可預期HSE, HAs, HPA可促進癌細胞凋謝死亡及抑制cell cycle progression。 從以上的結果，我們可能提出HSE, HPA及HAs可以預防及治療癌症，並說明其作用機轉，由於洛神花在國內容易栽培，產率高，而HSE, HPA及HAs也容易分離，因此，本研究成果將可應用於發展新藥。洛神花多酚酸花青素抗癌作用分離及分析Hibiscus Sabdariffa LinnaeusPolyphenoic acidAnthocyaninsTumor preventionDissociation and AnalysisProject