2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38637Establishment of cornea stem cell lines and analysis of their differentiation mechanisms (1/3) Although cornea is demanded for normal vision, there are many factors that may cause damage to cornea. In some circumstances, cornea will not repair by itself and will not be supplemented by culture and engraftment of contra-lateral limbal stem cells.With such, transplantation with donated tissues will be the only choice. However, donation is never readily available and many patients are put on the waiting list for years. The best solution for such dilemma will be establishment of permanent cornea stem cell lines and optimization of cornea in vitro and in vivo differentiation techniques.We have previously established some mouse cornea stem cell lines while studying the effect of Hedgehog protein on cornea repair.We found that Hedgehog protein with amniotic cell supports will facilitate growth of cornea stem cell lines in a spheroid mass. The cell masses were spit and sub-cultured for more than 24 passages without expression of cornea differentiation markers. This project aims to extend our previous findings by using the established mouse cell lines for optimizing transplantation therapy and for identification of novel factors in promotion of cornea differentiation. Furthermore, we will try to establish human cornea stem cell lines to facilitate treatment of corneal stem cell deficiency diseases in the future.建立眼角膜幹細胞株及其分化機轉之分析(1/3) 眼角膜為正常視覺所必需，但是很多因素都會導致受損，某些情況下眼角膜受損 無法自行修復，或是由另一側輪部幹細胞培養補充，此時移植治療成為唯一選 擇。但是移植需要他人捐贈，材料來源不穩定，往往讓接受者等上數年之久，解 決之道應該在於建立隨時可大量擴充培養的眼角膜幹細胞株，並且建立眼角膜幹 細胞於體內及體外分化之調控技術。我們先前於研究促進角膜修復機轉過程中， 意外發現以Hedgehog 蛋白加上羊膜細胞支援，可以讓眼角膜幹細胞形成球形細 胞團，並且可繼代培養，至今已經超過半年約24 個繼代，而且迄今尚無分化標 誌分子之表現。將此建立的細胞株移植於受損的小鼠角膜表面上，發現可以於 24 小時中快速修補受損的角膜。本計畫目標即在於利用已經建立的小鼠細胞 株，分析其培養及治療特性，並藉此分離出促進眼角膜分化因子，瞭解其分化機 轉，同時進一步建立人類的眼角膜幹細胞株，方便角膜細胞缺損患者之治療。Project