林巧雯Chiao-Wen Lin陳彥霖Yen-Lin Chen牙醫學系2026-03-172022-06-272022https://handle.ncl.edu.tw/11296/vynf92https://scholars.csmu.edu.tw/handle/123456789/5633在頭頸部的癌症中，口腔癌大約佔了百分之三十左右。時至今日，雖然手術、放射線治療以及化學治療已經是口腔癌的標準治療，但也因為口腔癌的復發率以及轉移造成對於口腔癌的治療以及預防都沒有一個較理想的成果。薑黃 (Curcuma longa) 早期被人們用來做為香料，染料等等功用，而其中的薑黃素 (curcumin) 被許多研究證實有抗菌、抗氧化、抗發炎、保肝和低血糖保護作用。越來越多的證據表明，薑黃素通過抑制腫瘤發生、進展、轉移、血管生成、化學抗性和放射抗性在預防和治療多種人類癌症中發揮保護作用。而許多薑黃異構物 (curcumin analog) 陸陸續續被研究證實可以改善薑黃生物吸收率代謝率差的缺點。在本實驗中，我們觀察到薑黃異構物 HO-3867 可以有效抑制 SCC-9 細胞存活率。而在流式細胞儀數據可以發現細胞在 sub-G1 期百分比增加。此外，我們發現 HO-3867 藉由活化 caspase8、caspase 9、caspase 3 和 PARP 的形成來誘導細胞凋亡，並且是透過 JNK1/2 路徑誘導細胞凋亡。我們的研究證實 HO-3867 是一種有效的抗癌劑，也相信藉由本實驗的結果可以幫助 HO-3867 在未來口腔癌的治療或預防上可以有更多的運用。Oral cancer accounts for about 30 percent of head and neck cancers.Although surgery, radiation therapy and chemotherapy are the standardtreatments for oral cancer, there is no ideal outcome for the treatment andprevention of oral cancer due to the recurrence rate and metastasis of oralcancer even today. Curcuma longa was previously used as a spice, dye, etc.Many studies have confirmed that curcumin has antibacterial, antioxidant,anti-inflammatory, hepatoprotective and hypoglycemic protective effects.Accumulating evidence suggests that curcumin exerts protective effects inthe prevention and treatment of various human cancers by inhibitingtumorigenesis, progression, metastasis, angiogenesis, chemoresistance,and radio-resistance. Many curcumin analogs have been successivelyconfirmed to improve the shortcomings of turmeric's poor biologicalabsorption rate and metabolic rate. In this study, we observed that thecurcumin analog HO-3867 inhibited the survival of SCC-9 cells. We foundthat HO-3867 effectively inhibited the growth of oral cancer cells. The flowcytometry data indicate that HO-3867 induces the sub-G1 phase. Moreover,we found that HO-3867 induces apoptosis by activating the formation ofcaspase 3, 8, 9 and PARP. We also found that HO-3867 induces apoptosisthrough the JNK1/2 pathway. Our results confirm that HO-3867 is aneffective anticancer agent, and we believe that the results of thisexperiment can help HO-3867 to have more applications in the treatmentor prevention of oral cancer in the future.口腔癌JNK薑黃異構物HO-3867細胞凋亡Oral cancerCurcumin analogApoptosisthesis