TING-HSING CHAO2026-10-162026-10-162025-01-01Pure ID: 15165090Pure UUID: 22e94c3b-6104-4a6f-b30a-9ba295ac05d110.2147/DDDT.S555188https://www.scopus.com/pages/publications/105021134731https://scholars.csmu.edu.tw/handle/123456789/24344Introduction: Peptic ulcer disease (PUD), including gastric and duodenal ulcers, is primarily caused by Helicobacter pylori infection and non-steroid anti-inflammatory drugs, with additional contributors like stress, certain foods, and alcohol. Proton pump inhibitors (PPIs) are effective but with adverse effects, prompting the exploration of alternative therapies. Hydrogen-rich coral calcium (HRCC) is a novel antioxidative agent that exerted anti-oxidative and anti-inflammatory activities; however, the effects of HRCC on PUD were still obscure. Methods: The gastroprotective effects of HRCC were investigated in a mouse model of PUD, and macroscopic score was used to investigate the effects of HRCC on PUD. In addition, the expression of the pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), C-C motif chemokine ligand 2 (Ccl2), and interleukin-6 (IL-6) by real-time quantitative polymerase chain reaction. In addition, Western blots were used to evaluate the expressions of anti-oxidative enzymes, including catalase, glutathione peroxidase-1 (GPx), and superoxide dismutase (SOD). Results: HRCC attenuated ulcer severity induced by ethanol and hydrogen chloride ex vivo (n = 6). In addition, long-term treatment of HRCC for seven-day reduced ethanol and hydrogen chloride-induced ulcer formation (n = 6). Moreover, we found that HRCC treatment decreased immune cell infiltration, the gene expressions of pro-inflammatory cytokines (n = 6), and enhanced antioxidative enzyme levels (n = 3) in stomach of the animals in vivo. Conclusion: HRCC has potential as a gastroprotective agent with anti-oxidative and anti-inflammatory activities, and its application may provide an alternative for treating PUD.7enjournal article