2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37437Liver disease is not only the major health problem of world wide, but also is one of the most important disease in Taiwan. Chronic hepatitis and liver cirrhosis are listed at the top of Taiwan's ten major cause of death. Hepatoma is also one of the most common malignant tumors for men and women. Therefore, to understand more about the mechanism of the development of liver disease, and to find out the way of early detection are one of the main focus of present research. Some evidences indicate that the activities of metalloproteinase (MMPs) are related to the development of liver cirrhosis and hepatocellular carcinoma. Accordingly, it is possible to know better about the cause and the mechanism of liver disease via the study of the relationship between the activities of MMPs of liver patients' sera and the pathological stages. Furthermore, the serum activity of MMPs could be used as assistant marker for the early diagnosis of liver malfunction. However, there are only few papers been published that investigated this topic, and they showed contradictory results. The purpose of the project is going to use the technique of zymography to look into the connection between the MMPs and the progression of liver disease; and to evaluate the possible application of this technique as serum marker. The sera of 60 subjects will be collected for each of the following groups: normal, HBe antigen carrier, HBs antigen carrier, HCV antigen carrier, chronic hepatitis (B type and C type) patients, acute hepatitis patients, liver cirrhosis patients and hepatoma patients. The activity levels of serum MMP-2 and MMP-9 will be analyzed by zymogram protease assay. Then, the protease activities will be statistically compared between groups, and the relationships among other serum markers and the protease activity will also be statistically analyzed.肝病不僅是世界性的疾病，亦是現今國人的大患，慢性肝炎與肝硬化向來是台灣居民十大死因之一，肝癌則是男性首位癌症死因，女性的第三位癌症死因。因此肝病的研究不僅是我國的既定政策，亦是海內外科學家努力研究的重要課題之一。雖然如此，至今對肝病的形成機制仍不甚明瞭，尤其是在治療與早期診斷方面，仍無法有所突破。 某些證據顯示metalloproteinase (MMPs)活性的改變可能和肝硬化或肝癌的形成有關，所以藉由探討患有不同時期肝病之病人其血中MMPs的改變，將有助於了解肝病的成因與機制；再者，MMPs的活性可能作為另一血中標記來輔助傳統的診斷。可是至今相關的研究並不多，且為國外的文獻，對此主題也無一致的看法，因此台灣本土的相關研究應儘快展開，以加強台灣的肝病研究與防治。 本計畫將藉由zymography的技術深入探討MMPs與肝病發展過程兩者之間的關係，以另一角度了解肝病的成因機制；並評估以MMPs活性當作血清中新標記的可行性，將其與現今較為通用的檢驗方式相互比較，以找出適當的檢驗程序，以達到早期診斷與預警的目的。 HBe、HBs和HCV抗原帶原者，B型與C型慢性肝炎患者，急性肝炎病人，肝硬化病人、肝癌病患與正常人等組各60名的血清收集後，以zymogram protease assay方法分析血清樣本中MMP-2與MMP-9的活性。之後，以統計軟體分析各組間MMPs的活性以及其他血液生化值的差異，找出每一組其生化值和腫瘤標記與MMPs活性的相關性。金屬蛋白分解酵素肝臟疾病酵素活性電泳分析MetalloproteinaseLiver diseaseZymographyProject