2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38729Activity-dependent reflex plasticity is the efficacy of a reflex varies depending on the ongoing neural activity. Central sensitization, which is characterized by a dynamic enhancement of the responsiveness of capsaicin-sensitive C-fiber to noxious stimulation following injury or inflammation, is a form of reflex plasticity that is presumed to underlie post-inflammatory hyperalgesia and tactile allodynia. Capsaicin-sensitive afferent fibers arising from the viscera and peripheral tissues express the transient receptor potential vanilloid subfamily member 1 (TRPV1). Activation of the TRPV1 receptor dynamically upregulates glutamatergic N-methyl-D-aspartate (NMDA) receptors expression on the spinal dorsal horn neurons and therefore elicits post-injury or post-inflammatory central sensitization. The uterus is one of the important reproductive organs within the pelvic cavity, and is a major source of female pelvic pain. Immunohitochemical studies demonstrated capsaicin-sensitive primary afferent fibers innervate rat uterine cervix, and activation of these fibers caused by injury, infection or malignancy induces obstetrical/gynecological neuropathetic pain and post-inflammatory hypergesia via central sensitization. A recent study investigating the pelvic-urethra reflex has demonstrated mechanical distension at the ureter induced a cross-organ interaction to modulate the pelvic-urethra reflex activity, however, so far, there is no adequate investigation upon the hypothesis that irritation of uterus may adversely influence the physiological activity of the lower urinary tract. Therefore, the first purpose of this study was to determine whether pharmacological stimulation of the uterus using capsaicin instillation in to the uterine horn might affect the function of the lower urinary tract in a cross-organ interaction manner. On the other hand, the level of estrogen dramatically remodels the physiological conditions of the uterus. If activation of the nociceptive C-afferent fiber arising from the uterus do cross-sensitize the lower urinary tract, whether the cross-organ sensitization may change with exposure to various levels of the estrogen will be an interesting question to be explored. The second purpose of this study was, therefore, to compare and contrast the cross-organ sensitization between the uterus and pelvic-urethra reflex activity at different stages of estrus cycle. The extracellular signal-regulated kinase 1/2 (ERK1/2), a mitogen activated protein kinase (MAPK), is activated by extracellular stimuli to mediate glutamatergic NMDA-dependent reflex plasticity. However, the role of ERK activation in the cross-organ sensitization has been yet to be established. We investigate the relationship between ERK activation and the cross-organ sensitization in the activity-dependent SRP as well as the intracellular protein kinase involved using immunoblotting methods.反射的強度依傳入活動而改變的現象稱為反射塑性。在受傷或是發炎時，辣椒素敏感性C纖維受刺激的機動性增加，稱為中央敏感性(central sensitization)，此依反射塑性推測與發炎後之過度疼痛有關。臟器及周邊組織含有TRPV1接受器，此會提升辣椒素產生的傳入纖維之敏感性。當TRPV1接受器被活化時會造成在脊髓背角神經上的glutamatergic NMDA接受器表現且引起損傷後即發炎後的中央敏感性。 子宮位於骨盆腔內，為女性骨盆疼痛之主要來源。經免疫組織化學的研究證明，在老鼠的子宮頸，其初級傳入纖維對於辣椒素具有敏感性，且這些纖維會因為外傷、感染或惡性腫瘤造成生產的/婦科的神經性疼痛以及發炎引發中央敏感性。在先前骨盆-尿道反射研究，證明輸尿管的機制性擴張會引起臟器間的交互作用，且調節骨盆-尿道反射活性，然而到目前為止尚無足夠的研究資料來說明。因此本次研究的首要目的為：測定在子宮上給予藥理學上的刺激，將辣椒素灌注進入子宮角會影響下泌尿道而引起臟器間交互作用。 另ㄧ方面，生理性週期會產生不同的雌激素的濃度。不同濃度的雌激素對於疼痛C纖維所造成的影響以及對於子宮與下泌尿道的臟器間交互作用成為一個有趣的論題。自本實驗的第二個目的為探討在不同階段的動情周期當中，比較子宮及骨盆-尿道反射活性之臟器間的敏感性。 細胞外訊息調節激酶(ERK1/2)為一絲裂原活化蛋白激酶(MAPK)，細胞外訊息調節激酶(ERK1/2)在細胞外被刺激可調節為glutamatergic NMDA依賴性的反射塑性。然而，細胞外訊息調節激酶(ERK)對於臟器間敏感性的活性目前尚未被證實。而我們則調查此兩者對於SRP的活性依賴性以及使用免疫轉漬法來看細胞內的蛋白激酶。Project