MAN-JUNG HUNG2026-10-162026-10-162010-09-01Pure ID: 15364168Pure UUID: 307afa4f-28ea-43ec-b5da-90ee19316c7310.1007/s00192-010-1168-3https://www.scopus.com/pages/publications/77956532075https://scholars.csmu.edu.tw/handle/123456789/29512Introduction and hypothesis Mesh-augmented reconstructive surgery for pelvic organ prolapse (POP) does not meet clinical expectations. A tissue-engineered fascia equivalent needs to be developed. Methods Human vaginal fibroblasts (HVFs) from 10 patients were characterized in vitro. Eligible HVFs and a biodegradable scaffold were used to fabricate a fascia equivalent, which was then transplanted in vivo. Results The cultured HVFs were divided into high (n=6) or low (n=4) collagen I/III ratio groups. Cells of the high-ratio group exhibited significantly higher proliferation potential than those of the low-ratio group (P<0.05). A fascia equivalent was made with HVFs of the high-ratio group. In the subsequent animal study, a well-organized neo-fascia formation containing HVFs could be traced up to 12 weeks after transplantation. Conclusions Our results suggest that a tissue-engineered fascia could be developed from HVFs in vitro and in vivo, which might be an effective treatment for POP in the future.9enjournal article