2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38585Diabetic bladder dysfunction (DBD) is a most common complication of diabetes mellitus (DM), but the mechanisms involved and treatment options are limited. The aim of this study is to examine the effect of electroacupuncture (Ea) at Zusanli (ST-36) acupoints on temporal changes in bladder function in streptozotocin (STZ) induced diabetic rats. Ea (100 Hz, 0.5mA, 1.5-2ms) is applied to bilateral Zusanli (ST-36) acupoints under conscious condition for 30 min every day following the induction of DM. We hypothesize that long-term Ea-treatment at Zusanli acupoints has therapeutic effects on DBD. Therefore, the present study is designed to find the molecular mechanisms involved in causing DBD, and to determine the effects of Ea-treatment on time-dependent changes in bladder function. The urodynamic parameters, bladder neuronal components, detrusor muscle contractility, the ratio of the smooth muscle myosin heavy chain isoforms SM2-to-SM1, actin isoform expression, thin filament-associated proteins expression in detrusor myoctyes are examined in female age matched control (with needling at Zusanli without stimulation), STZ-DM and STZ-DM-Ea treated Wistar rats 1, 4, 8, 12, 16 and 20 weeks after diabetes induction with STZ. Cystometry, immunohistochemistry, isometric contraction study, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot analysis, two-dimensional electrophoresis and immunofluorescence staining methods are done to identify our hypothesis. Ea should be considered as an alternative method for improving hyperglycermic stress induced with STZ in rats.We hope such a therapeutic technique has potential in clinical practice.糖尿病膀胱功能異常(diabetic bladder dysfunction, DBD) 是最困擾糖尿病(DM) 病患的問題之一，但目前對DBD 的致病機轉仍所知有限，也仍缺乏適當治療方法。因 此本實驗的目的主要探討長期在動物的足三里穴位(Zusanli acupoint) 進行電針刺激 (electroacupuncture, Ea)，觀察對streptozotocin (STZ)-誘發DBD 的影響。 將實驗動物分成控制組(未注射STZ，穴位進針但不通電刺激)、STZ-DM 動物組 及STZ-DM-Ea 處理三組，誘發DM 24 小時後每天在STZ-DM-Ea 動物兩側足三里穴 進行電針刺激(100 Hz, 0.5mA, 1.5- 2ms) 30 分鐘。在誘發DM 後第1, 4, 8, 12, 16, 20 週，每組各取5 隻動物進行實驗：第一年監測誘發糖尿病後的不同週數膀胱功能時間 性的變化，應用膀胱壓力描記術(cystometry) 記錄動物的尿路動力參數(urodynamic parameters)，並比較在初期便給予重複性及長期電針療法的效應。第二年探討膀胱組織 的型態及功能：以組織免疫染色方法觀察STZ-DM 膀胱的型態及神經支配變化；測試 STZ-DM 逼尿肌束收縮力對不同致效劑的反應；分析STZ-DM 逼尿肌的收縮性蛋白肌 凝蛋白(myosin) 及肌動蛋白(actin) 異構物的表現量，並與電針刺激組動物比較。第 三年利用real time PCR 及二維電泳方法，觀察比較細絲相關蛋白Caldesmon、 Tropomyosin、及actin 基因及蛋白層次表現量的變化；游離及培養逼尿肌細胞，利用 免疫多重染色方法及共軛焦顯微鏡觀察細胞內細絲相關蛋白的表現及空間分佈，比較 這些蛋白與myosin 在空間上的互動關係，以期對DM 造成膀胱逼尿肌型態重組及功 能異常的分子機制及電針產生療效的機轉有更進一步了解。 藉由本實驗設計進一步了解電針刺激足三里穴對DBD 具有療效的分子機轉，建立 與臨床應用情境相近的穴位刺激模式，期能對為DBD 所苦的病患提供更有效的替代療 法。糖尿病膀胱功能異常足三里穴電針刺激膀胱壓力描記術細絲相關蛋白肌凝蛋白逼尿肌Diabetic bladder dysfunctionelectroacupunctureZusanlimyosincystometrythin filament-associated proteindetrusor muscleProject