HWEI-YAN TSAI2026-10-162026-10-161991-01-01Pure ID: 15283487Pure UUID: 6349a496-8a84-46cf-9a49-a89f0db0252210.1016/S0021-9673(01)88773-2https://www.scopus.com/pages/publications/0025980726https://scholars.csmu.edu.tw/handle/123456789/34276The relatively slow kinetics of formation of the electroactive Cu(II)-peptide complexes from larger (>6 amino acids) peptides requires relatively high temperature and long reaction times for a postcolumn reactor. The precolumn incubation of bradykinin, Tyr8-bradykinin and insulin A chain with biuret reagent for 20 min at 60°C leads to the formation of biuret complexes which can be subjected to chromatography in acidic or basic eluents. These complexes are detected electrochemically with a sensitivity similar to the Cu(II)-(ala)3, complex (1 nC/pmol at 1.0 ml/min). The influence of the column-packing material on the electrochemical detector response of the Cu-peptide complexes has also been studied.6enjournal article