張菡馨Han-Hsin Chang朱芳怡Fang-Yi Chu營養學系2026-03-249999-12-3120202020-07-28https://handle.ncl.edu.tw/11296/kwh2tnhttps://scholars.csmu.edu.tw/handle/123456789/7134腸道發炎性疾病是21世紀嚴重的公共衛生問題，根據流行病學研究調查，其發生率在亞洲地區正在快速上升中，且台灣已經將其列入健保的重大傷病之一。在過去的研究中對於各種米糠產品以及其中成分硫辛酸（α-lipoic acid）和穀維素（γ-Oryzanol）改善腸道發炎性疾病的差異，以及在疾病發生的前後時間點進行飲食補充的效果是否相同，並沒有相關文獻進行比較。因此本研究以結腸灌注DNBS（dinitrobenzene sulfonic acid）誘導小鼠結腸炎，來探討市售米糠產品－包含米糠粉，2種不同品牌之米糠油以及其中功能性成分α-lipoic acid和γ-Oryzanol的預防和治療效果。實驗第一階段針對用於配製DNBS的溶劑酒精之濃度進行探討，我們發現50EtOH（50%酒精）相對於30EtOH（30%酒精）和PBS會在遠端結腸造成明顯的發炎細胞浸潤，所以接著使用30EtOH配製DNBS，並30EtOH作為對照組，第二階段總共分為30EtOH 組（30EtOH）、DNBS組（DNBS）、米糠預防組（RBP）、米糠油預防組（RBOP）、全穀油預防組（RBGOP）、α-Lipoic acid預防組（LAP）、 γ-Oryzanol預防組（ORYP）、米糠治療組（RBT）、米糠油治療組（RBOT）、全穀油治療組（RBGOT）、α-Lipoic acid治療組（LAT）及γ-Oryzanol治療組（ORYT），實驗為期25天，於第22天進行結腸灌注，預防組全程餵食補充飲食；而治療組於結腸灌注後隔天開始餵食補充飲食，所有組別於實驗第25天犧牲。 實驗結果發現，相較於30EtOH，DNBS出現明顯體重減輕，而LAP則有較輕微的趨勢，在組織切片中發現，DNBS造成遠端結腸組織產生嚴重的病理現象，包含發炎細胞浸潤、杯狀細胞流失、潰瘍和隱窩膿腫的出現，而預防組對其改善的效果有比治療組更好的趨勢，其中RBP降低了MPO的表達：RBOP降低了CD44的表達：RBGOP降低了NF-κB、COX-2和CD44的表達：LAP和ORYP則降低了NF-κB、COX-2、MPO和CD44的表達，另外LAP也降低了細胞增殖標記蛋白Ki-67的表達。綜合以上結果，對於DNBS誘導的小鼠結腸炎組織病理現象，預防性補充α-Lipoic acid和γ-Oryzanol的改善效果最明顯，而市售之米糠產品的補充則有改善的趨勢，而治療性的補充整體而言不如預防的效果。Inflammatory bowel disease has emerged as a public health challenge in the 21st century. Epidemiological studies suggest that incidence might be rising rapidly in Asia, and Taiwan has listed it as one of the catastrophic illnesses in the National Health Insurance. There is no study to compare the difference in the improvement of intestinal inflammation between various rice bran products and its components, α-lipoic acid and γ-Oryzanol, and whether the effect of dietary supplementation the same before or after the occurrence of the disease. Therefore, we investigated the preventive and therapeutic effects of commercially available rice bran products,including rice bran powder, two different brands of rice bran oil, and the functional components－α-lipoic acid and γ-Oryzanol using DNBS-induce colitis in mice. In the first stage of the experiment, we explored the concentration of ethanol as a solvent for DNBS. Compared with 30EtOH ( 30%Alcohol ) and PBS, 50EtOH ( 50%Alcohol ) will cause obvious inflammatory cell infiltration in the distal colon. Therefore, DNBS is prepared with 30EtOH and 30EtOH used as a control group. In the second stage, mice were divided into 30EtOH group (30EtOH), DNBS group (DNBS), Rice bran prevention group (RBP), Rice bran oil prevention group (RBOP), Rice bran and germ oil prevention group(RBGOP), α-Lipoic acid prevention group (LAP) ), γ-Oryzanol prevention group (ORYP), Rice bran treatment group (RBT), Rice bran oil treatment group (RBOT), Rice bran and germ oil group (RBGOT), α-Lipoic acid treatment group (LAT) and γ-Oryzanol treatment group (ORYT).The experiment lasted for 25 days, with rectal administration on the 22nd day, all prevention groups were fed the supplementary diet throughout the course; while all treatment groups were fed the supplementary diet the next day after rectal administration, and all groups were sacrificed on the 25th day. The results showed that, the severe weight loss caused by DNBS only alleviated in the LAP group. Based on histopathology scores, DNBS induced severe mucosal inflammation, with inflammatory cell infiltration, loss of goblet cells and increased appearance of ulcerations and crypt abscesses,the improvement effect of the prevention group tended to be better than that of the treatment group.RBP reduced the expression of MPO, RBOP reduced the expression of CD44, RBGOP reduced the expression of NF-κB、COX-2 and CD44, LAP and ORYP reduced the expression of NF-κB、COX-2、MPO and CD44, and LAP reduced the expression of the cell proliferation marker protein Ki-67.These results suggest that preventive supplementation of α-Lipoic acid and γ-Oryzanol has the most obvious improvement effect in the DNBS-induced histopathological examination, and the supplementation of commercially available rice bran products has a trend of improvement , while the therapeutic supplementation is not as effective as prevention as a whole.腸道發炎性疾病米糠米糠油硫辛酸穀維素Inflammatory bowel diseaseRice branRice bran oilα-Lipoic acidγ-Oryzanolthesis