謝易修陳霈霓Yih-Shou, Hsieh 謝易修Pei-Ni ,Chen 陳霈霓林君香Jiun-Siang Lin生化暨生物科技研究所2026-10-289999-12-312010https://handle.ncl.edu.tw/11296/5crabghttps://scholars.csmu.edu.tw/handle/123456789/35517過去文獻指出，氧化型低密度脂蛋白( oxidative modification of low-density lipoprotein;ox-LDL)在粥狀動脈硬化形成中扮演著重要的角色。續隨子(Euphorbia lathyris)是天然的中草藥，目前已知具有抗發炎(anti-inflammation)和抗癌 (anti-tumor)等生物活性，然而對於續隨子是否會抑制粥狀動脈硬化形成及其詳細的作用機制到目前為止還不清楚。在本篇論文中，我們首次證實續隨子具有抑制LDL的氧化作用並且保護氧化型低密度脂蛋白對人類血管內皮細胞(HUVECs) 所造成的傷害。針對抑制LDL氧化實驗中，利用relative electrophoretic mobility (REM) shift assays、electrophoresis of ApoB fragmentation、lipid peroxidation assay，證實續隨子可有效抑制硫酸銅所誘導LDL的氧化。在氧化型低密度脂蛋白對人類血管內皮細胞所造成的傷害實驗中，續隨子同時也可抑制氧化型低密度脂蛋白誘發人類臍靜脈內皮細胞的損傷，主要是透過抑制氧化型低密度脂蛋白所破壞粒腺體膜電位(mitochondrial membrane potential) 及抑制細胞凋亡過程中所釋放出的Bax並使得 pro-apoptosis protein Bcl-2之活化，此外，續隨子同時也可降低氧化型低密度脂蛋白誘發人類內皮細胞的細胞內導致ROS的產生。綜合以上結果，續隨子或許可應用於臨床上保護氧化型低密度脂蛋白所造成內皮細胞的功能失調及傷害。The oxidative modification of low-density lipoprotein (LDL) is thoughtto have a central role in the pathogenesis of atherogenesis.Euphorbia lathyris extract (ELE) is a natural constituent of plants and ELE, has several anti-inflammation and anticancer biological effects. However, its protective effects on LDL oxidation and endothelial injury induced by oxLDL remain unclear.In this study, we evaluated the antioxidative activity of ELEand how ELE rescues human endothelial cells (HUVECs) from oxidized LDL (oxLDL)-mediated dysfunction. The antioxidative activity of ELE was defined by the relative electrophoretic mobility of oxLDL, fragmentation of ApoB,and malondialdehyde production via the Cu2+-mediated oxidation of LDL.ELE also inhibited the generation of ROS and the subsequent mitochondrial membrane potential collapse by oxLDL in HUVECs. Our results suggest that ELE may protect LDL oxidation and prevent oxLDL-induced cellular dysfunction.thesis