徐再靜余豐益Tsai-Ching HsuFeng-Yih Yu郭靜怡Jing-Yi Guo生化微生物免疫研究所2026-10-299999-12-3120202020-08-20https://handle.ncl.edu.tw/11296/93r8xghttps://scholars.csmu.edu.tw/handle/123456789/36532人類微小病毒B19 (B19V)與許多臨床疾病相關，包含血液疾病、胎兒水腫和自體免疫疾病等。許多研究指出感染人類微小病毒B19患者會加重原先病情與併發症的產生。目前臨床上用於檢測人類微小病毒B19的檢驗方法主要利用酵素免疫連結法偵測血清中專一的B19V-IgM及B19V-IgG抗體或聚合酶連鎖反應方法偵測B19V-DNA，然而這些診斷試劑缺點是需要耗費時間及儀器設備才能得到結果。因此，希望建立一套膠體金免疫層析法提供簡易的操作以快速檢測人類微小病毒B19。目前經由B19V抗原和B19V血清標準品進行評估，顯示我們所建置B19V抗原及抗體膠體金免疫層析法快速檢測平台，可提供快速且專一性B19病毒抗原或IgM及IgG抗體檢測結果。Human parvovirus B19 (B19V) is associated with many clinical diseases, including blood diseases, fetal edema and autoimmune diseases. Previous research indicates that patients infected with human parvovirus B19 will aggravate the patients' condition and complications. At present, the clinical diagnostic assay for B19V mainly uses an enzyme-linked immunosorbent assay to detect specific B19V-IgM and B19V-IgG antibodies in serum or polymerase chain reaction to detect B19V-DNA. However, the disadvantages of these diagnostic methods for B19V are time consuming and needed professional equipment to get the results. Therefore, in this study, we hope to establish a set of colloidal gold immunochromatographic assay for B19V in order to provide a rapid and easy-to-use detection approach. We use B19V antigen and serum standard controls to evaluate the colloidal gold immunochromatographic test for B19V. The results show that this detection platform can provide specific test for B19V antigen and antibody and distinguish between negative and positive samples.人類微小病毒B19膠體金免疫層析法human parvovirus B19colloid gold immunochromatographic assaythesis