2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37777Salvia pleveia R. Brown is one of common health protective herb in Taiwan. According to the traditional herb studies, it has been provided with the therapeutic ability for soret-hroat,hemoptysis and hepatitis (1-4). In the hepatoprotective studies, the extracts of the different kinds of Salvia pleveia R. Brown have also been evidenced to have the anti-inflammatory effect in the CCl4-treated mices(7). Many reports have indicated that liver fibrogenesis and cirrhogenesis are mainly due to the activation of hepatic stellate cells(HSCs) (23). When normal liver cells are injured by virus or alcohol, HSCs will be transformed to activation (such as increase of fibrogenesis and the growth rate and the secretion of cytokine), and finally cause liver dysfuction, fibrosis and cirrhosis. So, to inhibit HSCs activation will be the best choice to prevent liver fibrosis and cirrhosis. In our laboratory, the primary data showed that the extracts of Salvia pleveia R. Brown inhibited the serum-induced morphological changes and cell proliferation in (HSCs)Fig. (1,2). However, whether the activation of HSCs is really inhibited by Salvia pleveia R. Brown is still unconfirmed. Thus, this experimental design will further investigate the mechanism of the inhibitory effect of Salvia pleveia R. Brown on the serum-induced HSCs activation, and also will screen the medical effect of the different types of Salvia pleveia R. Brown collected from different areas in Taiwan. This experiment will be performed in one year. First, Salvia pleveia R. Brown will be collected from the north,Midst,south and eastern part of Taiwan and will be extracted by water-boiling-water extraction. HSCs separated from Wistar male rats will be treated with different concentrations(5,1,0.1,0.01mg/ml)of the extracts of Salvia pleveia R. Brown from different area(north,midst,south and eastern part)and different types, and then observed cell morophology and measure cell growth rates. In the same time, to confirm the inhibitory effect the expressions of cytokine (IL-6)and pro-collagen a2 mRNAs will be detected by using RT-PCR analysis, and the marker of the fibrosis (a-SMA) and the intracellular signalling (MAPK/MAPK-phosphorylation and MAPKK/MAPKK- phosphorylation by Western Blot will be also screened. These results not only will provide the evidence of the anti-inflammatory effect of Salvia pleveia R. Brown in liver, but will also set up a novel cellular model of the anti-inflammatory effect in liver for screening nature medicines.七層塔是台灣的常用民間保健草藥之一，根據中草藥的文獻指出七層塔有治療咽喉腫痛、咳血及肝炎等療效 (1-4)。另外，在保肝方面的研究指出利用四氯化碳處理的小鼠加入不同品種的七層塔萃取液均被證實有抑制發炎的現象 (7)。 研究報導指出肝星狀細胞（hepatic stellate cells, HSC）活化是造成肝纖維化及肝硬化形成的主要因素 (23)。當正常肝細胞受到病毒或酒精等因素傷害時，肝星狀細胞開始轉型活化 (例如纖維化、增加生長速率及分泌發炎激素等現象)，最後導致肝功能失常、纖維化和肝硬化。因此如何抑制肝星狀細胞的活化，將是有效防止肝纖維化及肝硬化的最佳選擇。我們實驗室初步實驗發現七層塔萃取液有抑制雄鼠的肝星狀細胞的型態改變和生長速率（圖一、二）。然而七層塔是否具有抑制肝星狀細胞活化的能力，仍然未知。因此本實驗計畫將深入探討七層塔萃取液抑制肝星狀細胞活化的作用機制，也同時偵測台灣不同區域內不同七層塔品種的藥效。 本實驗為一年期計劃。首先將台灣北、中、南及東部四區內的七層塔全草進行傳統水萃取。然後收集Wistar雄鼠內的肝星狀細胞進行初代培養，並處理不同地區（北、中、南及東部）、不同品種及不同劑量（5、2、1、0.1、0.01mg/ml）的七層塔萃取液，觀察細胞型態及細胞增殖速率的現象。同時採用RT-PCR來偵測肝星狀細胞內細胞激素（IL-6）及原膠元蛋白a2 mRNA表現，和利用西方點墨法測定訊息傳遞路徑（MAPK/MAPK-磷酸化及MAPKK/MAPKK-磷酸化）的抑制情形。此項結果不僅將證明七層塔具有抑制肝發炎的功效，同時也建立天然藥物抑制肝臟發炎反應的細胞模式。七層塔肝星狀細胞細胞激素Salvia pleveia RBrownhepatic stellate cellsIL-6Project