2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38794Aflatoxin B1 is a mycotoxin that is produced by Aspergillus species. It is commonly found in food, cereal products, and feed in which cause toxic effects and cancer in human and animal. The International Agency for Research on Cancer (IARC) has classified aflatoxin B1 as 1A group of human carcinogen. Development of rapid detection methods for aflatoxin B1 is important issue in food safety. We have developed the specific antibodies against aflatoxin B1 and established the enzyme-linked immunosorbent assay (ELISA) to analyze the aflatoxin B1 in foods and feeds. Recently, there are several reports on aflatoxin contamination in animal feeds which causing canine illness and the use of expensive imported ELISA kits for aflatoxin B1 detection is increasing. Therefore, the objective of this proposal is focused on development of a ELISA kit for aflatoxin B1 using the specific antibodies that developed by us. The ELISA kit was based on an antibody coated onto the ELISA plate. The whole assay time can be finished in 30 min by adjusting the aflatoxin B1-horseradish peroxidase concentration. This ELISA kit is planning to technical transfer to a biotechnology company in Taiwan. We expect the biotechnology company could establish a Taiwan brand name for aflatoxin B1 ELISA kit. This kit can be used not only in Taiwan but also can be exported to use in foreign country.黃麴毒素B1(aflatoxin B1)主要由Aspergillus 屬等黴菌所產生的小分子致癌毒素，泛存於各類食品、榖物與飼料產品中，食用遭受黃麴毒素污染的食品與飼料會導致人類或動物許多疾病及癌症的生成。國際癌症協會已將黃麴毒素B1歸類為1A族是人類的直接致癌物，因此黃麴毒素B1對於人類及動物體健康有高度的威脅，因此開發各式快速檢測分析法來分析調查各類食品中與飼料中的黃麴毒素B1的污染是一重要課題。目前本實驗室針對黃麴毒素B1已經生產獲得具有專一性極佳之多株抗體，並分別建立非直接與直接競爭型酵素免疫分析法來分析調查各類食品與飼料中的黃麴毒素B1。近年來，由於黃麴毒素B1汙染食品與飼料的問題日趨嚴重，購買使用黃麴毒素B1免疫分析套組日漸增加，從國外進口來的黃麴毒素B1酵素免疫分析套組又非常昂貴，因此本計畫將以自行生產之黃麴毒素B1專一性抗體進行黃麴毒素B1酵素免疫分析套組之開發，將2小時可以完成的檢測分析時間，藉由抗體首先吸附在ELISA plate上，再調整適當的毒素與酵素接合物的濃度，縮短至25~30分鐘內來完成檢測結果，此技術計畫移轉給國內的生技公司，期能建立台灣生技公司黃麴毒素B1免疫分析套組自有品牌，除了可以提昇產業檢驗試劑生產技術供國內業界使用，並可外銷至其他國家，對於生技產業將有相當大的助益。黃麴毒素B1酵素免疫分析套組aflatoxin B1antibodyELISA kitProject