KAI-LI LIU2026-10-162026-10-161996-01-01Pure ID: 15451769Pure UUID: 0c065d10-7a09-45fa-93bf-23a1b81218f8https://www.scopus.com/pages/publications/33749099578https://scholars.csmu.edu.tw/handle/123456789/32417Conjugated linoleic acid (CLA) has been shown to inhibit mouse skin tumor promotion To evaluate whether CLA was able to be incorporated into keratinocytes, the uptake of I4C-conjugated linoleic acid (CLA) was compared to the incorporation of l4C-linoleic acid (LA) and 14Carachidonic acid (AA) over time The mouse keratinocyte cell line, HEL30, was grown in serum free media, 0.1% albumin and O.luCi/ml I4Cfatty acid (LA, CLA or AA) for various time points (0.5-24 hr) Incorporation of the fatty acids was maximal by 12 hr and approximately 85-90% exogenous radiolabeled fatty acid was incorporated into cells by 24 hr Incorporation of 4C-CLA into keratinocytes was slightly lower compared with uptake of I4C-LA or I4C-AA The incorporation I4C-I.A and I4C-CLA into phospholipid fractions was similar The majority (-57%) of I4C-LA or C-CLA was incorporated into phosphatidylcholine (PC) with phosphatidylethanolanie (PE) and phosphatidylserine/ phosphatidylinositol (PS/PI) containing 15%-27%. In contrast, 14C-AA incorporation was evenly distributed into PC (29%), PE (29%) and PS/PI (39%) To determine competition of CLA and LA for incorporation, cells were cultured in serum free media with I4C-LA 0 IfiCi/ml and unlabeled CLA (0, 1,4. 16 ng/ml) for 12 hr. At various doses, CLA inhibited I4C-LA incorporation into phospholipids. In summary, CLA and LA were incorporated into keratinocyte phospholipids in a similar and competetive manner (Supported by ACS IRG 1735).enjournal article