YUAN-YEN CHANGYI-HSIEN HSIEHSHUN-FA YANGHUI-WEN LIN2026-10-162026-10-162019-03-01Pure ID: 15159465Pure UUID: be9edf57-e0f9-455b-b8c4-d6ac795c251610.1002/tox.22686https://www.scopus.com/pages/publications/85057846881https://scholars.csmu.edu.tw/handle/123456789/26924Kaempferol is a flavonoid with anticancer and anti-metastasis activity in different cancer-cell lines. However, the underlying mechanisms by which kaempferol acts on human retinal pigment epithelial (ARPE-19) cells remain unclear. In this study, we demonstrated that kaempferol inhibited migration and invasion in ARPE-19 cells at non-toxic dosages. We discovered that kaempferol obviously reduced the enzyme activity and protein expression of matrix metalloproteinase-2 by increasing the phosphorylated levels of extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathways. Additionally, ERK1/2-specific inhibitor PD98059 significantly reversed kaempferol's inhibitory effects on migration and expression of MMP-2 in ARPE-19 cells. Overall, our results are the first to demonstrate that kaempferol is capable of inhibiting cell migration by targeting ERK1/2 signaling in human retinal pigment epithelial cells.7enjournal article