2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37585Hibiscus sabdariffa Linne (Malvaceae), an attractive plant believed to be native to Africa is cultivated in Sudan and in eastern and Taiwan. It has been reported to contain a number of flavonoids including gossypetin, hibiscetin and quercetin. In vitro experimental studies have shown that administration of the aqueous extract produces anti-inflammation, hypotensive and hypocholesterolemic. In spite of the wide use of H. sabdariffa extract (HSE) in folk medicine for treatment of various diseases, there are no reports in the literature on their anti-atherosclerosis activity. The aim of this study was to evaluate the effects of an aqueous extract obtained from Hibiscus sabdariffa (HSE) on the (1) susceptibility of LDL to oxidation and on plasma lipid in rabbits affected by experimental atherosclerosis induced by the intake of high levels of saturated fat and cholesterol; (2) inhibition of ox-LDL-induced apoptosis in cultured endothelial cells, the mechanisms of anti-apoptosis of HSE in endothelial cells would be studied to determine whether the apoptotic signals and gene expression are involved; (3) inhibition of proliferation and cell cycle progression and induced cell apoptosis in aortic smooth muscle cells (ASMC). The molecular mechanisms of the anti-proliferative and apoptotic effects of HSE in ASMC would be investigated.The results of the these studies may be used to demonstrate the effects of HSE on the preventive cardiovascular diseases by (1) antilipidemia and inhibit LDL-oxidation; (2)inhibit ox-LDL-induced apoptosis in endothelial cells; (3) anti-proliferation in ASMC. These results also provide HSE as a health food for preventing cardiovascular disease.洛神花盛產於熱帶地區及台灣東部與南部，具抗血壓及抗發炎等功效，由於其成份含有原兒茶酸及類黃酮素（包括gossypetin, hibiscetin 及quercetin等）都為強抗氧化劑，且都含於水萃取物中。因此本研究探討對（1）洛神花水萃取物（HSE）降血脂及抑制低密度脂蛋白氧化作用，進一步探討（2）抑制氧化型低密度脂蛋白（ox-LDL）誘導內皮細胞apoptosis（可能與atherosclerotic pathogenesis 有關）及（3）探討抑制 aortic smooth muscle cell （ASMC）增生之作用是否與cell cycle arrest 及apoptosis 有關。第一年之計劃擬探討HSE抑制血脂肪及抗LDL之氧化作用，分別在cell free system 及endothelial cell中以thiobarbituric acid reactive substances （TBARS）及electrophoretic mobility（EM）觀察LDL氧化情形；進一步以兔子動物模式誘導之atherosclerosis來確認HSE 降血脂及降低ox-LDL形成；由於ox-LDL誘導內皮細胞apoptosis，可能與atherosclerosis或thrombosis相關。從以上的結果，我們預期HSE有降血脂、抗LDL氧化作用。因此，HSE可發展為一種預防心血管疾病之保健食品。洛神花水萃取物氧化型低密度脂蛋白降血脂作用凋謝死亡抑制增生Hibiscus Sabdariffa Extracts (HSE)LDL OxidationHypolipidemiaApoptosisCell Cycle ArrestAnti-proliferationProject