JIUNN-LIANG KOFENG-YIH YU2026-10-162026-10-162020-10-01Pure ID: 15179627Pure UUID: da9adfe9-d219-4a95-86a5-c8fe60d9d8b610.3390/toxins12100637https://www.scopus.com/pages/publications/85092271787https://scholars.csmu.edu.tw/handle/123456789/24361A two-analyte immunochromatographic strip (immunostrip) was developed for the simultaneous detection of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. Protein conjugates (AFM1-ovalbumin (OVA) and CAP-OVA) and goat anti-rabbit IgG were respectively drawn on nitrocellulose membrane as two test lines (T1 and T2) and a control line (C). The immunostrip was dipped into a well that contained a 200 µL milk sample, 5 µL AFM1 antibody-gold conjugates, and 8 µL CAP antibody-gold conjugates; the whole assay was completed in 15 min and the results could be interpreted visually or using a reader. This immunostrip has cut-off levels of 0.1 ng/mL and 0.5 ng/mL for AFM1 and CAP, respectively. Analysis of CAP and AFM1 in milk samples revealed that data from the immunostrip test agreed closely with those obtained from ELISA. The two-analyte immunostrip is a rapid way for on-site simultaneous detection of AFM1 and CAP in milk.enjournal article