徐再靜HSU, TSAI-CHING郭家瑋KUO, CHIA-WEICHIA-WEIKUO醫學研究所碩士班2026-10-292029-08-0920242024-07-15https://handle.ncl.edu.tw/11296/wb2mfkhttps://scholars.csmu.edu.tw/handle/123456789/36710肺纖維化是由感染、長期吸入有害化學物質以及間質性肺部疾病等多種因素所引起的臨床嚴重肺部疾病。發生肺纖維化時，會出現乾咳、呼吸困難、運動耐受性下降等症狀，嚴重時甚至可能危及生命。間質性肺部疾病一般是指引起肺間質發炎和浸潤的疾病，包括環境粉塵暴露引起的間質性肺病和自體免疫性間質性肺病(如系統性硬化症（SSc）和類風濕性關節炎（RA）]。目前臨床上雖然有延緩間質性肺部疾病的藥物，但治療效果有限。回顧文獻發現，許多臨床間質性肺病的病例與人類微小病毒B19（B19V）感染有關，但其機制並不清楚。B19V 是微小病毒科無套膜 ssDNA 病毒，傳播途徑主要為呼吸道感染，其基因主要編碼兩種結構蛋白VP1和VP2以及非結構蛋白NS1。本研究利用氣管滴注博來黴素所誘導的小鼠肺纖維化模型，探討B19V病毒蛋白對肺纖維化的影響。研究結果顯示B19V非結構蛋白NS1會加劇博來黴素誘導的小鼠肺纖維化情形。而透過此動物模型之建立讓我們能更清楚的瞭解B19V感染對於肺臟纖維化的生理發展及病理影響。Pulmonary fibrosis is a severe lung disease caused by infections, prolonged exposure to harmful chemicals, and interstitial lung diseases. The condition leads to symptoms such as dry cough, shortness of breath, and decreased exercise tolerance, and in severe cases, it can be life-threatening. Interstitial lung diseases encompass a range of conditions that cause inflammation and scarring of the lung tissue, including those triggered by environmental dust exposure and autoimmune disorders like systemic sclerosis (SSc) and rheumatoid arthritis (RA). While there are drugs available to slow down the progression of interstitial lung diseases, their effectiveness is limited.Interstitial lung disease is associated with human parvovirus B19 (B19V) infection, but its molecular mechanisms are unclear. B19V is a non-enveloped ssDNA virus of the parvovirus family, primarily transmitted through respiratory infection, with its genome mainly encoding two structural proteins, VP1 and VP2, and a non-structural protein, NS1. This study utilized a bleomycin-induced mouse model of pulmonary fibrosis to investigate the effects of B19V viral proteins on pulmonary fibrosis. The experimental results showed that B19V non-structural protein NS1 may exacerbate bleomycin-induced pulmonary fibrosis in mice. This animal model can help us better understand how B19V infection impacts the progression and development of pulmonary fibrosis.人類微小病毒B19B19Vthesis