2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38770Development of chemotherapeutic drugs for treatment of variety cancers is an important issue nowadays. Cytotoxicity assay is the first step of new drug development. These assays measure the effect of a drug on the growth of a population of cells and the end point is an estimate of cell number. One of the most commonly used approaches is to determine the surviving cell numbers indirectly by tetrazolium dye (MTT) reduction. A drawback of the assay is that it does not distinguish between a cytotoxic and a cytostatic effect. The quartz crystal microbalance (QCM) will be used to monitor cell growth during cell cultures in this proposed research. The curve of frequency response for variety cell lines, such as prostate cancer cell (PC-3), ovarian cancer cell (SKOV3), and colon cancer cell (HT-29) will be established. The QCM response is based on the coupling effects of loading mass and viscoelastic properties around the crystal surface. The frequency change of QCM during the cell culture will be confirmed with traditional method of MTT and fluorescenct microscope. The cell-population pictures at various time periods and the absorption change of MTT are two of most used methods for measuring cell growths. Both frequency and resistance responses may provide real-time information on the coverage of cancer cells on electrode. We will correlate the information of traditional methods and the information of QCM. The material of electrode on quartz crystal, such as gold and silver will be evaluated for the better adhesion of the cells. Selected proper anticancer drugs will be studied to investigate the feasibility of the QCM system by culture of the cancer cells in the presence and absence of the testing drug. Some of selecting herbal medicines will be evaluated for the cytotoxic effects.癌症每年均高居先進國家十大死亡之ㄧ，是目前威脅人類健康和生命的頭號殺手， 無論是製藥界或是學術界均積極地找尋有效的治療方式。抗癌藥物發展的第一階段就 要先利用癌細胞培養測試新藥對癌細胞生長的抑制效用(即藥物活性,drug activity 或稱 細胞毒性測試)。於實驗終了測試存活的細胞數，常用之檢測原理為活細胞粒線體中的 琥珀酸脫氫酶能使外源性MTT 還原為水不溶性的藍紫色結晶甲臢（Formazan）並沉積 在細胞中，而死細胞無此功能。二甲基亞碸（DMSO）能溶解細胞中的甲臢，用酶聯 免疫檢測儀在500nm 或570nm 波長處測定其光吸收值，可間接反應活細胞數量。此 方法的缺點是無法分辨細胞毒性或細胞自然死亡。本研究是要利用QCM 的技術, 監測 細胞培養時之生長曲線，如prostate cancer cell (PC-3), ovarian cancer cell (SKOV3), and colon cancer cell (HT-29)等，QCM 頻率之改變與吸附在電極上之質量及晶片表面之黏 滯性有關，因此本研究亦會利用MTT 及螢光顯微鏡照像雙重確認QCM 之信號，建 立信號與細胞生長之相關性(correlation)。QCM 的頻率與阻抗的改變可同時提供細胞 生長的即時訊息。 本研究亦會探討不同電極材料, 如金電極或銀電極對細胞貼附的影響，第一年除了 基本信息相關性之建立，並將利用已上市之抗癌藥物對適當之細胞系（cell line）做藥 物活性測試之可行性探討，第二年將利用此系統評估中草藥之抗癌活性。石英微量天平細胞培養藥物活性quartz crystal microbalance (QCM)cell culturedrug activityProject