2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37772Trichlorfon a kind of organophosphate insecticide, has been widely used in agriculture as a broad spectrum insecticide based on its action as an inhibitor of acetylcholine esterase. Prochloraz is widely used as an agriculture fungicide. The action od imidazole fungicide is based on the inhibition of cytochrome P-450-dependent step in ergosterol synthesis. In the present project, we hypoesized that chronic exprosure of trichlorfon and prochloraz around skin might induced tumor promotion and absrpotion toxicity, which could cause alteration of serum biochemical in the 2 month, 4 month and 6 month treatment. To investigated the toxicity of trichlorfon and prochloraz in rats, following work will be finished: (1) analysis serum biochemical level; (2) patholgenic examination; (3) marker of tumor promotion assay (inflammation, hyperplasia, ornithaine decarboxylase, myloperoxidase activity, H2O2 and hyperplasia). The results of these studies may be used to demonstrate the organ toxicity and tumor promotion of trichlorfon and prochloraz in rat skin exposure 6 month.現今,將選以三氯松及撲克拉農藥為第一年度之研究重點,本實驗室將擬用大白鼠背部塗抹三氯松及撲克拉類農藥施以市售建議量1倍及5倍處理劑量,六個月慢性長期接觸來觀察農藥毒理.期間追蹤試驗其第2及4個月大鼠之生化檢驗及臨床血液學檢驗,在試驗期滿6個後犧牲做病理學觀察,並將血液收集再加生化檢驗及臨床血液學檢驗,以完成為期6個月的血液及生化功能之追蹤,在病理方面,擬觀察犧牲後大鼠取皮膚及肝、腎與胰臟做細胞型態及臟器受損評估.藉由長期生化檢驗及病理學觀察,建立是否對於大鼠長期接觸國內市售三氯松及撲克拉類藥物售否會誘發體內臟器之傷害及皮膚病理變化,進而誘使各種實質性細胞損傷之評估,或促進體內細胞病變現象產生,期望為現今市售trichlorfon及prochloraz農藥建立對於使用者或與農藥生產相關人員長期接觸農藥的醫學健康傷害評估,提供未來國內農藥使用及管理規範之依據,並在未來利用此模式為其他國內農藥建立使用及管理規範之依據.Project