CHUN-I LEETSUNG-HSIEN LEE2026-10-162026-10-162023-12-01Pure ID: 15022053Pure UUID: bf093798-ce5a-48c1-a8ef-fc9593eebf8810.1038/s41598-023-31472-4https://www.scopus.com/pages/publications/85150313369https://scholars.csmu.edu.tw/handle/123456789/24466This study evaluated whether the concentration of biphasic O2 (5–2%) promotes the formation of qualified blastocysts (QBs) and euploid blastocysts and the probability of cycles with transferable blastocysts. The paired experimental design included a total 90 patients (180 cycles) without euploid blastocysts in previous monophasic O2 (5%) cycles were enrolled for an additional cycle of biphasic O2 (5–2%). In the biphasic O2 (5–2%) group, the QB rate (35.8%, 225/628) was significantly higher than that in the monophasic O2 (5%) group (23.5%, 137/582; p < 0.001). In addition, the euploid blastocyst number (0.5 ± 0.8) and the percentage of cycles with transferable blastocysts were significantly higher in the biphasic O2 (5–2%) group (57.8%, 52/90) than those in the monophasic O2 (5%) group (0 and 35.6%, 32/90, respectively; p < 0.01). Multivariable regression analysis also indicated that the QB rate and the probability of cycles with transferable blastocysts correlated with O2 tension (OR 1.535, 95% CI 1.325–1.777, and OR 3.191, 95% CI 1.638–5.679, respectively; p < 0.001). Biphasic O2 culture can be used as an alternative strategy to increase the euploid QBs and the probability of cycles with transferable blastocysts in patients with a poor prognosis.enjournal article