許立松莊雅如Ya-Ju生化暨生物科技研究所2026-10-289999-12-312011https://handle.ncl.edu.tw/11296/399rpkhttps://scholars.csmu.edu.tw/handle/123456789/35702大腸直腸癌是全世界第三大常見的惡性腫瘤，慢性腸炎是發生癌變的高危險族群。在免疫系統的調控過程中，IL-10為具有多種功能的細胞激素，可以直接促進發炎反應亦可抑制發炎反應，被認為在發炎反應的過程中扮演著相當重要的角色。基因多型性與許多疾病發生有關，許多研究顯示IL-10啟動子的單一核苷酸多型性與某一些癌症及自體免疫反應都有密切關係。本篇研究主要是探討IL-10啟動子區域上(-819) C/T單一核苷酸多型性是否與大腸直腸癌好發有關。實驗分析296個檢體，針對IL-10基因啟動子上-819位置的多形性進行分析，以聚合酶連鎖反應增幅出啟動子上包含-819位置的DNA片段，並以限制酶片段長度多型性分析方法來分析實驗族群中-819單一核苷酸多型性頻率分布。結果發現大腸直腸癌患者的IL-10 (-819) C/T genotype對實驗中的對照組（IIA：有疾病風險族群和IIB：無疾病風險族群）是有差異性存在；推測IL-10 (-819) C/T 單一核苷酸多型性與大腸直腸癌的好發可能是有相關性。另外，實驗結果也顯示C/C型與對照組的IIB族群有差異性，暗示C/C 單一核苷酸多型性與大腸直腸癌好發，可能有其潛在的關聯性。本研究的結果顯示IL-10啟動子區域之-819位置單一核苷酸多型性，似乎和大腸直腸癌好發有正相關。CRC is the 3rd most common malignancy worldwide. The individuals with chronic inflammatory bowel diseases are high risky populations of colon carcinogenesis. IL-10 is a multifunctional cytokine that induce or suppress inflammatory reaction which has been considered playing a key role in the regulation of inflammatory reaction mediated by immune system. Single nucleotide polymorphism (SNP) is related with disease arising has been proved. There are many studies indicated that SNPs on promoter region of IL-10 gene are highly associated with some cancers and autoimmune diseases. In this research, we study on the relationship between the SNP at IL-10 (-819) C/T and CRC arising. Total 296 individual samples were collected and analyzed the frequency distribution of SNPs at IL-10 (-819) by Polymerase Chain Reaction amplifying DNA fragment form promoter region then genotyping by Restriction Fragment Length Polymorphism in the study. The data were analyzed by Chi-square test (X2 test) and there was statistic difference at -819 C/T genotype between CRC and control group (IIA, with disease risks; IIB, without disease risks ) indicating that the IL-10 (-819) C/T SNP may be associated with CRC arising. In addition, the statistic difference was also indicating at C/C type between CRC and control IIB groups that indicates the potential association of C/C type and CRC arising. Overall, our results show the SNP at -819 on promoter region of IL-10 gene may be positively associated with CRC arising.thesis