2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38073The establishment of Dietary Reference Intakes of Taiwan was mostly based on the data of other countries. Therefore, it is very important to establish our local data for the revision of nutrient recommended intakes. The purpose of this study is going to assess vitamin B-6 status and requirement of 31-50 y men and women. Two hundred (men=100; women=100) healthy subjects' age ranged from 31-50 y are going to be recruited from the check-up unit of Chung Shan Medical University hospital. Height and weight are measured and body mass index is calculated. Nutrient intake was recorded using 24-h diet recall. Fasting venous blood and 24-h urine samples are obtained. Plasma pyridoxal phosphate, pyridoxal, urinary 4-pyridoxic acid, erythrocyte alanine (EALT-AC) and aspartate (EAST-AC) aminotransaminase activity coefficient measured are measured to assess vitamin B-6 status. Post methionine load homocysteine is also measured. Differences in nutrient intakes and biochemical values are compared by using Student t-test. Pearson correlation coefficient is conducted to assess the relationship between vitamin B-6 status indicators and intakes. Linear regression analysis of various vitamin B-6 assessment measures (dependent variables) and intakes (independent variables) are analyzed to determine mean inverse estimates of the vitamin B-6 requirement. To determine the estimated average requirements (EAR) of vitamin B-6 and 95% confidence intervals, extrapolations from linear regression analyses are made. The recommended dietary allowance is then calculated from EAR x 1.2.國人膳食營養素參考攝取量的訂定大都參考國外的相關資料，因此建立本土資料以作為政府機關訂定或修正國人營養素參考攝取量之參考是非常重要的。本研究計劃的目的是評估31 – 50歲男性及女性的維生素B-6營養狀況及訂定維生素B-6的建議攝取量。健康受試者將從中山醫學大學附設醫院家庭醫學部體檢科募集31 – 50歲男女性各100位。受測者的身高及體重都將測量並計算身體質量指數 (BMI)。以24小時飲食回憶法做營養素攝取的記錄，並且收集空腹血液、及24小時尿液。分析血漿磷酸比哆醛、比哆醛、尿液比哆酸、血球丙胺酸轉胺酉每;活性係數及血球天門冬胺酸轉胺酉每;;活性係數來評估受測者維生素B-6的營養狀況。另外甲硫胺酸負荷後的同半胱酸濃度也將一併分析。所得的研究資料以Student t-test 比較男性及女性的營養素攝取量及各項維生素B-6營養狀況指標值的差異性。Pearson correlation coefficients將用來評估維生素B-6攝取與各項維生素B-6生化指標值的相關性。以迴歸曲線(regression analyses)求得各項維生素B-6營養狀況指標值(y)與維生素B-6攝取量(x)的關係(例如：PLP = a x B-6攝取量+ b)，以外插法分別將各項指標值的切點帶入迴歸關係式以求得維生素B-6攝取量的平均需要量(EAR)及其95%的信賴區間值(confidence intervals)。取其各項指標值分別所求得的維生素B-6攝取量EAR的平均值x 1.2以求得維生素B-6的建議攝取量(RDA)。維生素B-6磷酸比哆醛比哆酸轉胺酉每活性係數平均需要量建議量vitamin B-6pyridoxal-5'-phosphatepyridoxic acidtransaminase activity coefficientaverage requirementrecommended dietary allowanceProject