李健群Chien-Chun Li張心瑜Hsing-Yu Chang營養學系2026-03-249999-12-312019https://handle.ncl.edu.tw/11296/h4qm3nhttps://scholars.csmu.edu.tw/handle/123456789/7099根據全球癌症最新數據GLOBOCAN 2018統計，乳癌為全世界婦女癌症發生率首位。其中三陰性乳癌因轉移及復發風險高，預後不佳成為導致乳癌患者死亡之主因。因此，如何降低三陰性乳癌生長及轉移成為目前各國致力於探討的議題。先前研究證實香蕉偽莖嫩芯萃取物(banana pseudostem tender core extract, BPTCE)具有抗氧化活性。本研究利用不同萃取方式之BPTCE，探討其對Hs578T三陰性乳癌細胞凋亡及轉移之影響。研究結果顯示，在凍乾(Freeze-dry, FD)、殺菁凍乾(Blanch and Freeze-dry, BFD)、榨汁(Squeeze, S)及殺菁榨汁(Blanch and Squeeze, BS)等四種不同萃取方式得之萃取物中，以殺菁榨汁萃取物處理後Hs578T細胞促凋亡蛋白表現最高。因此本研究後續採用殺菁榨汁香蕉偽莖嫩芯萃取物(BPTCE -BS)進行實驗。MTT分析細胞存活率結果顯示，隨著BPTCE-BS處理濃度(0, 100, 200, 400 μg/ml)的增加，Hs578T乳癌細胞死亡率隨之增加。細胞凋亡檢測結果發現，預處理200、400 μg/ml BPTCE-BS 24小時後，顯著誘發Bax/Bcl-2蛋白表現比例並促使Hs578T乳癌細胞凋亡。此外cleaved caspase-7及PARP cleavage蛋白表現亦隨BPTCE-BS處理濃度增加而顯著上升。透過粒線體分離實驗結果發現，粒線體中Bax/Bcl-2蛋白質表現比例在400 μg/ml的BPTCE-BS處理細胞20小時後達到最大值。Wound-healing assay結果顯示低濃度(100、200 μg/ml)的BPTCE-BS可降低Hs578T乳癌細胞移行能力。Boyden chamber assay結果顯示25 μg/ml的BPTCE-BS可降低Hs578T乳癌細胞侵襲能力。預處理不同濃度(0, 25, 50, 100, 200 μg/ml)的BPTCE-BS可降低MMP-9酵素活性及蛋白表現，抑制效果呈劑量關係。綜合上述結果可知，BPTCE-BS可透過啟動凋亡內在路徑促進Hs578T三陰性乳癌細胞凋亡，並藉由抑制MMP-9蛋白表現及活性降低癌細胞轉移。According to GLOBOCAN 2018, breast cancer is the most commonly diagnosed cancer in women worldwide. Triple negative breast cancer (TNBC), due to its propensity for recurrence and metastasis, is associated with poor outcomes, higher mortality rate and is more challenging to manage compare to other subtypes of breast cancer. Tremendous efforts have been directed towards understanding the pathways underlying TNBC progression and metastasis.BPTCE has been shown to have antioxidant properties in a previous study. The aim of this study is to investigate the effects of banana pseudostem tender core extract (BPTCE) on apoptosis, migration and invasion in Hs578T human TNBC cells. Among the four different extraction methods by using freeze-dry (FD), blanch and freeze-dry (BFD), squeeze (S) and blanch and squeeze (BS), treatment with blanched and squeezed banana pseudostem tender core extract (BPTCE-BS) significantly increased apoptotic protein expression. Results from MTT assay showed that BPTCE-BS dose-dependently decreased cell viability in Hs578T cells. Treatment with 200 and 400 μg/ml of BPTCE-BS significantly increased Bax/Bcl-2 protein expression ratio and apoptotic cell death. The protein level of caspase-7 cleavage and PARP cleavage were induced by BPTCE-BS in a dose-dependent manner. The expression ratio of Bax/Bcl-2 in mitochondrial fraction was dramatically increased after treatment with 400 μg/ml of BPTCE-BS for 20 hours. Wound-healing assay demonstrates that BPTCE-BS has a significant inhibitory effect on cell migration once the concentration reaches beyond 100 μg/ml. Results from boyden chamber assay showed that cell invasion significantly decreased after treatment with 25 μg/ml of BPTCE-BS. BPTCE-BS treatment also reduces MMP-9 protein expression and enzyme activity in a dose-dependent manner. These results indicate that BPTCE-BS is capable of inducing apoptotic cell death through the intrinsic apoptosis pathway. A decrease in cell migration and invasion is achieved by suppressing MMP-9 protein expression and enzyme activity in Hs578T human TNBC cells.香蕉偽莖嫩芯凋亡移行侵襲三陰性乳癌細胞banana pseudostem tender coreapoptosismigrationinvasiontriple negative breast cancer cellsthesis