許立松賴映晴Ying-Ching Lai生化微生物免疫研究所2026-10-299999-12-312017https://handle.ncl.edu.tw/11296/r539r5https://scholars.csmu.edu.tw/handle/123456789/36274大腸直腸癌是全世界第三大常見癌症，為台灣人十大癌症發生率之首。過去文獻指出，多種原因包括遺傳、高齡化、發炎性腸道疾病、代謝因素皆會導致大腸癌發生，而引發正常細胞癌化的最魁禍首是基因不斷的突變，它會使得細胞不受控制的生長，其中突變最常見的形式為單核苷酸基因多樣性。許多研究顯示，基質金屬蛋白酶家族在腫瘤的侵襲和轉移的過程中扮演著重要的角色，家族內由巨噬細胞分泌的基質金屬蛋白酶12（Matrix metallopeptidase 12, MMP-12）既可降解細胞外基質使腫瘤更容易轉移還可以促使血管抑素的釋放抑制腫瘤生長，在不同的癌症中MMP-12發揮了促進腫瘤發展或抑制的功能。目前，各種研究也證實MMP-12基因多樣性會影響著癌症的進展。綜合以上，為了瞭解MMP-12 A82G基因多樣性是否與大腸直腸癌相關，我們利用聚合酶連鎖反應增幅了MMP-12基因啟動子上-82位置的DNA片段，並以限制酶切割片段長度多樣性的方法分析了86個檢體中MMP-12 A82G基因多樣性。結果顯示，所有檢體中MMP-12（82）只出現了A/A基因型，由此可知台灣地區大腸直腸癌病患與MMP-12 A82G基因多樣性沒有相關性。Colorectal cancer is the third most common cancer worldwide, with its incidence rate ranked first among the top 10 cancer incidence rates in Taiwan. Previous literature has indicated various risk factors of colorectal cancer, including heredity, aging, inflammatory bowel disease, and metabolism. The primary cause of malignant cell formation is attributable to continual gene mutation, which induces uncontrolled cell growth. Of the various mutation types, single nucleotide polymorphism is the most common. Many studies have reported that matrix metalloproteinase (MMP) family members play a crucial role in tumor invasion and metastasis. Of these members, matrix metalloproteinase 12 (MMP-12) secreted by macrophages can both degrade the extracellular matrix to induce tumor metastasis and enhance the release of angiostatin to inhibit tumor growth; in other words, MMP-12 can either exacerbate or inhibit tumor development under different cancer scenarios. Various studies have verified the effect on MMP-12 polymorphism on cancer progression. According to the aforementioned assertions, this study was conducted to explore the correlation between the genetic diversity of MMP-12 A82G and colorectal cancer. Polymerase chain reaction was used to amplify the DNA segment at the -82 element of the MMP-12 promoter, and restriction fragment length polymorphism was employed to analyze the genetic diversity of MMP-12 A82G in 86 specimens. The results revealed A/A as the only genotype appearing in the specimens’ MMP-12 (82). Accordingly, no correlation was observed between the genetic diversity of MMP-12 A82G and colorectal cancer in Taiwan.基質金屬蛋白&#3723812單一核&#33527酸多樣性大腸直腸癌Matrix metallopeptidase 12Single-nucleotide polymorphismsColorectal Cancerthesis