許立松Li-Sung Hsu劉珊秀Shan-Hsiu Liu生化暨生物科技研究所2026-10-299999-12-312015https://handle.ncl.edu.tw/11296/k7397thttps://scholars.csmu.edu.tw/handle/123456789/36161在全世界癌症發生中大腸直腸癌已是常見惡性腫瘤之一；在男性排名第三位，在女性排名第二位。其中有多項危險因子﹕有大腸直腸癌家族史者、高油脂低纖維飲食、抽菸、喝酒、年齡、潰瘍性腸炎…等。有許多研究指出氧化壓力和許多疾病及癌症有關如肺癌、肝癌、大腸直腸癌等。本實驗之目的主要利用聚合酶連鎖反應－限制酶切割片段多型性分析的方法研究超氧化物歧化酶1（superoxide dismutase 1， SOD1）與台灣的大腸直腸癌相關性。我們分析 64位大腸癌患者其SOD之等位基因，和134位年齡相仿健康對照組，以RFLP方式偵測SOD135 A/C基因的多形性，結果產生三種不同片段﹕若產生71和207兩個DNA片段，則表示此SOD1基因型為C/C同型合子(Homozygous)；若只產生一個和278 DNA片段則表其為A/A同型合子(Homozygous)；若產生71、207、278三個片段則表示此基因型為A/C異型合子(Heterozygous)。結果顯示SOD1基因多型性在台灣大腸癌患者與對照組間並無明顯差異，皆為A/A同型合子(Homozygous)。Colorectal cancer (CRC) is one of common malignant tumors which ranked third in men and ranked second in female. There are a number of risk factors such as family history of CRC, high-fat low-fiber diet, smoking, drinking, age, and ulcerative colitis for development of CRC. Many studies have pointed out that oxidative stress can cause many diseases and cancers such as lung cancer, liver cancer, CRC. The main purpose of this study using the polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP) analysis method to study the association of superoxide dismutase 1 (SOD 1) 35A/C polymorphism and colorectal cancer in Taiwan relevance. We analyzed 64 patients with colorectal cancer and 134 age-matched healthy control group. RFLP assay may result in three different segments: if generating two DNA fragments 71 and 207 bp, it means SOD1 genotype C / C with homozygous; if only showed a 278 bp and it means A / A with homozygous; if it is three fragments 71,207,278 bp, this genotype is A/ C heterozygous. The results showed that SOD1 gene polymorphism did not differ significantly between Taiwan colorectal cancer patients and controls as all are A / A homozygous genotype.超氧化岐化&#372381大腸直腸癌SOD1CRCthesis