徐成金Cheng-Chin Hsu楊紋青Wen-Ching Yang營養科學研究所2026-03-242004-06-292004https://handle.ncl.edu.tw/11296/6z8934https://scholars.csmu.edu.tw/handle/123456789/6700山藥為一般食物來源及中國傳統藥用植物。在台灣，山藥通常被當作滋補保健功能的營養品。近來，許多研究指出必需脂肪酸及蛋白質攝取不平衡可能導致憂鬱症的發生。有鑑於日本與台灣食用山藥的處理方式不同；因此，本研究目的在評估山藥在生食與熟食不同濃度處理下對ICR雄鼠血清中必需脂肪酸濃度及總蛋白質含量的影響。實驗以大汕二品系、民間長紅與日本山藥三種山藥，分別以生食與熟食處理凍乾山藥粉取代控制組飼料(AIN-76)中10%、20%飼料，老鼠飼育2、4、8週後犧牲；並以高效能液相層析（HPLC）測定血清及山藥中必需脂肪酸包括：Linoleic acid（LA:18:2n-6）、α-Linolenic acid（ALA：18:3n-3）、Arachidonic acid（AA:20:4n-6）及Oleic acid（18:1n-9）的濃度。結果顯示經由皂化過後熟食大汕組中ALA及AA的含量有顯著高於其他組別，皂化步驟較類似人體生理代謝效應。熟食大汕山藥具有提高小白鼠血清中Linolenic acid濃度的效果。而熟食處理過後的山藥組長期餵食（八週），具有提高血清總蛋白質濃度的效果。因此，我們可以進一步探討熟食大汕山藥是否具有預防或改善憂鬱症的功效。The yam is a common source of food and herbal medicine in China. In Taiwan, it is usually used as a tonic nourishment. Taiwan and Japan have apparently different way for consuming dioscorea. Recently, many research findings that imbalance in the ratio of the essential fatty acids (EFAs) and total protein level correlate to major depression. The aim of this study was to evaluate the effect of uncooked and cooked Dioscorea spp(Wild yam), as well as the influence of dioscorea concentrations on EFAs concentration and total protein level on serum in adult ICR male mice. In this experiment, three species dioscorea（Dioscorea alata L., Dioscorea alata L. Var. Purpurea M. Pouch. and Dioscorea japonica L.）differentiate between uncooked and cooked lyophilized yams powder which were replaced 10% and 20% of AIN-76 control diet, respectively. Mice were sacrificed after two, four, and eight weeks feeding, respectively. By high pressure liquid chromatography（HPLC）measured EFAs, linoleic acid（LA：18:2n-6）, linolenic acid（ALA：18:3n-3）arachidonic acid（AA：20:4n-6）, and Oleic acid（18:1n-9）on serum in mice and lyophilized yams powder. Results showed that ALA and AA concentration in cooked Dioscorea alata L. group was significantly higher than other groups via saponification step, which was similar the effect of physiology metabolism in human. Dioscorea alata L. can enhance ALA concentration on serum in mice. Cooked Dioscorea alata L. long period of time fed can raise total protein level on serum in mice. Therefore, we will further investigate whether cooked Dioscorea alata L. can prevent or improve major depression.山藥必需脂肪酸憂鬱症Dioscorea sppEssential fatty acidsMajor depressionthesis