2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38654We have tested different gene expression of cumulus cells from advanced and young infertility women. Limited by sample size and physiology age, we cannot evaluate gene expression on cumulus for infertility women completely. In this study we just inclusion criteria patients for treated in vitro fertilization (IVF) with intracytoplasmic sperm injection (ISCI). In order to evaluate the effects of cumulus cells on the quality of oocytes and embryos, we used real-time quantitative PCR to determine the levels of gene expression.We also test telomere length to evaluate the effect on real reproductive age of cumulus and embryo development.We select several genes, from our previous chip data and other reports, that are involved directly may effect cellular aging and embryo development, LH receptor, FSH receptor, Progesterone receptor, PTGS2 (Prostaglandin-endoperoxide synthase 2), HAS2 (hyaluronan synthase -2), GREM1(gremlin 1), CYR61(cysteine-rich-angiogenic inducer-61), CUL1 (cullin 1), RPS28, c-Kit ligand, LIF(leukemia inhibitory factor), bFGF (basic fibroblast growth gactor), BMP-4(bone morphogenetic proteins 4). This study are separated three year for administration, the first and second year, we will detect telomere length and gene expression of cumulus cells and analysis the relationship between these data and cleavage stage embryo development.We will analyze the relationship between gene expression data and blastocyst stage embryo development on the third years.We hope to find some genes for embryo development marker for selecting good embryos to improve embryo implantation rate.在先前的研究計畫中，我們曾利用基因晶片篩選出高齡與年輕婦女卵泡周圍的顆粒 細胞基因表現之差異，但是由於採量數量過小及未客觀的評估生殖係統的老化情形，為 充分結合基礎研究與臨床應用，我們將觀察不同年齡患者在進行常規試管嬰兒合併單一 精蟲顯微注射的療程中，採集卵子周圍欲丟棄的卵泡周圍的顆粒細胞進行可能影響卵子 成熟的基因以及可能影響胚胎發育的基因包括評估分裂期與囊胚期胚胎品質的指標基 因，另外配合端粒表現量的測定分析這些基因在高齡與年輕婦女表現量的差異期望可以 提供臨床治療的參考。本計畫將分三年期進行，第一年與第二年進度為收集常規試管嬰 兒合併單一精蟲顯微注射的療程且將進行第三天胚胎植入的個案，進行激素接受體基因 表現量分析卵泡發育的因子分析，以及進行端粒長度鑑定，經受試者同意調閱其胚胎發 育的病例紀錄等資料予以紀錄存檔，觀察顆粒細胞端粒長度即激素接受體是否可應用於 胚胎發育的參考。第二年則將第一年的檢體依胚胎發育分級，分析可能影響胚胎發育的 的因子包括PTGS2, HAS2, GREM1, CYR61, CUL1, RPS28, Kit ligand, Lif, bFGF, BMP-4 等因子，以即時定量PCR 分析基因表現量，期望找出指標基因供篩選胚胎的指標。第 三年則針對收集常規試管嬰兒合併單一精蟲顯微注射的療程且將進行第五天囊胚期胚 胎植入的個案的顆粒細胞進行基因表現量分析。本研究期望找出足以作為卵子及胚胎發 育及篩選依據之指標基因以提高不症患者之胚胎著床成功率。Project