2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37516key words: Female lung cancer, oil smokes, 8-hydroxyguanine, hOGG1. OGG1 gene have been found in E. coli and Yeast. It is a glycosidase gene which involved in the repair process to remove the oxidative DNA damage, 8-hydroxyguanine (8-OH-G). Recent study has demonstrated that human was also to have OGG1. Our previous results indicated that 8-OH-G was formed in human lung adenocarcinoma CL-3 cell treated by oil smokes from frying fish (FOSC). At least three lung carcinogenic compounds, benzo[a]pyrene (B[a]P), 1,6-dinitropyrene (1,6-DNP), and 2-amino-3,8-dimethylimidazo[4,5-f]qunoxaline (MeIQx) have been found to exist in FOSC. Taken together, the oxidative DNA damage and bulky DNA adduct in human lung cells were indeed resulted by FOSC. In animal model, FOSC has been demonstrated to have pro- carcinogenic potential from the results of p53 and MDM2 protein overexpression, the induction of high PAH-DNA adduct levels, and pathological changes in lung tissues. In this project, we attempt to investigate the role of oxidative stress induced by FOSC in female lung carcinogenesis. The correlation between the amounts of 8-OH-G and OGG1 gene expression in non-tumor tissues surgical resected from female lung cancer patients will be evaluated by HPLC and PCR respectively. In addition, the difference of OGG1 gene expression between tumor and non-tumor lung tissues was also investigated in this study. We hope that our results would be helpful to understand the role of oxidative stress by oil smokes in female lung carcinogenesis.最近研究發現人類有類似酵母菌、大腸桿菌之參修補氧化性傷害所造之8-hydroxyguanine (8-OH-G)的OGG1基因。而本研究 室已在八十六年度的計劃中，發現烹調油煙能引起人類肺癌細胞株產生8-OH-G，同時也以LC-MS確定其產生，流行病學 的許多證據都顯示台灣女性肺癌的發生和烹調油煙具有相關性。而本研究室在執行本計劃的各年度計劃中，陸續發現煎 魚油煙中含有至少三種能在動物實驗上引起肺腫瘤之致癌物，分別為benzo[a]pyrene (B[a]P), 1,6-dinitropyrene (1,6-DNP), 和 2-amino-3,8-dimethylimidazo[4,5-f]qunoxaline (MeIQx)，且能在CL-3 細胞造成DNA adduct。因此可確定油煙具有引起人類肺細 胞的基因毒性。同時在動物實驗上，我們也證實煎魚油煙萃取物具有致癌潛力。本年度計畫擬由烹調油煙所造成之氧化 性傷害方面來了解油煙在引起女性肺癌發生上的可能性。因此擬由修補8-OH-G的基因在肺癌患者之腫瘤和非腫瘤組織中 的表現和肺組織中8-OH-G之含量的相關性，並已過去建立之女性肺癌患者暴露油煙之問卷資料，來推暴露油煙在引起女 性肺癌之可能性性因此本年度計畫擬完成下列研究工作： 一、 比較肺癌患者腫瘤組織和非腫瘤組織中OGG1的基因表現，以了解肺癌致腫瘤過程，OGG1基因可能扮演之角色。 二、 比較暴露油煙的廚師和非廚師之血液淋巴球之OGG1基因表現，以了解OGG1是否能作為暴露油煙的生物指標。 三、 分析肺癌患者非腫瘤組織中的8-OH-G之含量，以了解氧化性傷害，在肺組織之OGG1的基因表現的相關性。 四、 以肺癌細胞株暴露油煙在雌性素存在和不存在下，來了解OGG1的表現和8-OH-G 產生之相關性以及雌性素的影響。 總之，本計劃之晚成將有助於了解烹調油煙在氧化性傷害方面對女性肺癌發生的角色。女性肺癌烹調油煙8-hydroxyguaninehOGG1 基因CL-3Project