2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38632In our previously grand NSC 95-2320-B-040-027 from the National Science Council and Academic Sinica, for estimate myocardial ischemia or ischemia-reperfusion (I/R) injury, we ligated left coronary artery 30 min or 5min ischemia following 30 min reperfusion to investigate the antiarrhythmic effects of luteolin in the rats under myocardial ischemia and I/R injury. We found that luteolin is a potent cardioprotective agent with antiarrhythmic effect in myocardial ischemia and I/R injury rats. During the same period, pretreatment with luteolin also decreased (lactate dehydrogenase) LDH levels, an indicator of cell damage, and nitric oxide (NO) production in the carotid blood. Luteolin is one of the most wildly presented flavonoids in many kinds of fruits and vegetables. There are several literatures had been reported that luteolin possess antineoplastic, antihepatotoxic and antiosteoporotic effects. An appropriate intake of luteolin with anti-allergic activities by inhibited IL-4 production. Luteolin also had been shown to protect DNA against free radicals in human melanoma HMB-2 cells. In addition, luteolin showed a marked cytoprotective capacity in PC12 cells against H2O2 injury induced predominantly apoptotic death by the ability to modulate intracellular signals promoting cellular survival. Recently, Theoharides et al. reported luteolin with anti-inflammatory activities because pretreatment with luteolin totally blocks mast cells stimulation and T cell activation. Mast cells are involved in allergic and inflammatory reactions because they are located perivascularly and secrete numerous proinflammatory cytokines. In this study, we will evaluate the cardioprotective effect of luteolin on myocardial infarction on rats subjected to 1 hr ischemia and 3h reperfusion. In addition, we will estimate the creatine kinase (CK) and LDH activity in plasma to correlate with the myocardial infarction treated by luteolin. In addition, the antioxidant activity, downregulation of NO production, and anti-inflammatory activities of luteolin may have beneficial effect on the model of myocardial ischemia reperfusion injury. Furthermore, for investigation the mechanism of protective effect of luteolin against myocardial ischemia reperfusion injury, in this project, we test the role of nitric oxide, antioxidant effect, and anti-inflammatory activity on the protective effect of luteolin on the myocardial ischemia reperfusion injury本人95 年度的國科會計畫中(NSC 95-2320-B-040-027)，利用大白鼠心 臟遭受三十分鐘冠狀動脈缺血或是五分鐘冠狀動脈缺血而後再灌注三十分鐘 的實驗動物模式，研究luteolin 對抗心律不整的心臟保護作用，研究發現 luteolin 是一個很有效的心臟保護劑，在心肌缺血以及缺血再灌注傷害時具有 抗心律不整的作用，在相同的區間內，luteolin 還可以減少頸動脈血的血漿中 細胞損傷因子LDH 的活性以及一氧化氮的產生。 Luteolin 是一種黃酮類，廣泛存在很多種的水果和蔬菜中，已經有很多 文獻報導luteolin 具有抗腫瘤作用、抗肝臟毒性以及抗骨質疏鬆的作用，攝取 適量的luteolin 可以抑制IL-4 的產生，而具有抗過敏的活性，luteolin 在human melanoma HMB-2 cells 也表現出可以保護DNA 對抗自由基的傷害，此外， luteolin 在PC12 cells 也表現出明顯的細胞保護作用利用調節細胞內訊息傳遞 對抗H2O2 傷害所引起明顯的細胞凋亡，最近，Theoharides 等人報導預先給 予luteolin 可以完全拮抗mast cells 刺激以及T cell 活化，所以具有抗發炎活 性，mast cells 位在內臟周圍分泌許多種proinflammatory cytokines，所以和過 敏以及發炎反應相關。 在本計劃中，我們將大白鼠冠狀動脈結紮造成心肌缺血一小時後再灌注 三小時引發心肌梗塞，評估luteolin 對抗心肌梗塞的心臟保護作用，同時評估 血漿中細胞損傷因子creatine kinase (CK)以及LDH 的活性，並和luteolin 對於 心肌梗塞的保護作用做相關性評估。此外，因為luteolin 的抗氧化作用、抑制 NO 的產生，以及抗發炎活性對於心臟缺血再灌注傷害應該是有益的，所以在 本計劃中，我們會研究luteolin 對抗心肌缺血再灌注傷害的心臟保護作用機 轉，評估血漿中一氧化氮的量，以及心肌中一氧化氮合成酵素蛋白質以及 mRNA 的表現量、抗氧化活性、以及抗發炎作用在luteolin 的心臟保護作用中 所扮演的角色。Luteolin心臟保護心肌梗塞抗氧化一氧化氮抗發炎LuteolinCardioprotectionMyocardial infarctionAntioxidantNitric oxideAntiinflammationProject