2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38042In this study, we plan the following three procedures to investigate the chemical constituents of humic acids in soils and well waters in Taiwan, especially the complexing characteristic of arsenic-humate. (1) The samples from soils and well waters are primarily extracted humic acids, fulvic acids and humans by acid/alkaline solution and resin preconcentration respectively. They are analyzed by elemental analyzer, UV-VIS spectroscopy, Infra-Red spectroscopy and capillary electrophoresis, which is based on the mode of capillary electrochromatography and show well resolution by the fine adjustment of temperature, voltage and modifiers to the ACN mobile phases. These results will establish the composition distribution map of humic materials in Taiwan. (2) The above samples are finely extracted by supercritical fluid extraction and following the same analytical methods to get best structure resolution. As regards the determination of arsenic species, using reversed electroosmotic flow separation mode by the modifier addition to change the Zeta potential of stationary phases and phosphate base buffers as mobile phases is expected to resolve the both organic and inorganic arsenics. The resultant data should be checked by atomic absorption spectrum. (3) In aqueous system, the properties of equilibrium constants, complex capacities and thermodynamics under various pH values, ionic strengths and temperatures are obtained by the methods of discrete single site and Schubert’s ion-exchange. In aqueous-solid system, the absorption curves, partition coefficients and maximum capacities for humic materials are first surveyed. Then the equilibrium properties of arsenics and humic materials with known amount loaded on solid matrices are also obtained. Finally The arsenic-humate complexes in synthetic matrices and real soils are eluted under simulated environmental conditions as nature and analyzed its constituents by our established methods. These will offer much valuable information of distribution area and concentration, as well as risk assessment.本研究擬進行下列三大步驟，逐步瞭解台灣地區土壤及井水中腐植酸化學成 分，尤其與砷之錯合特性：(一)土壤及井水樣品分別以酸、鹼性溶液及樹脂濃縮 吸附以初步萃取出樣品中腐植酸、黃腐酸及腐黑質，以元素分析、紫外-可見光 譜、紅外線光譜及毛細管電泳分析針對腐植酸及黃腐酸 (或其酸解產物) 分析濾 液，其中毛細管電泳分析法是以毛細管電層析機制，選擇ACN 為主的動相溶液， 並調整溫度、電壓及添加劑等其它影響參數，以求最佳的分離解析度，此結果 將可建立台灣地區土壤中腐植質之成分分佈地圖；（二）以超臨界流體直接精萃 上述樣品，並且進行同樣分析工作，以求得更佳的結構解析。而砷物種分析可 先將樣品先吸附或鉗合濃縮後，再以逆電滲透流機制，選擇磷酸鹽為主的動相， 並且選擇適當修試劑改變靜相的Zeta 電位，以同時解析出無機及有機砷物種， 此結果可與原子吸收光譜相較。(三) 以discrete single site 及Schubert’s ion-exchange 方法求出液相系統中腐植酸在不同的pH 值、離子強度以及溫度下 水溶液的平衡常數、錯合容量及熱力學常數。在液-固系統中先求取腐植酸的吸 附曲線、分配係數及最大吸附量，再將已知腐植酸吸附量的固態基質與砷物種 錯合平衡，同樣地求取其在各種平衡條件下的吸附曲線及平衡常數，最後將砷 吸附後之基質或原始土壤樣品經各種可能的天然模擬條件下溶析出，分析析出 液之腐植酸或砷物種之組成，提供可能流佈的範圍及濃度，並進一步作為風險 評估的之基礎資料。土壤井水腐植酸砷毛細管電泳超臨界流體萃取soilwell waterhumic acidarseniccapillary electrophoresissupercritical fluid extractionProject