2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38753Obesity is an important topic in the world of public health and preventive medicine. Obesity is closely associated with a state of chronic, low-grade inflammation characterized by abnormal cytokine production and activation of inflammatory signaling pathways in adipose tissue. Recently, studies have showed that antioxidant functions may inhibit the obesity-related inflammation. A typical instance has been proven as N-acetylcysteine (NAC) and resveratrol, which attenuates TNF-α induced changes in secretion of adipocytokines. However, the data concerned with other naturally occurring antioxidants on obesity regulation is limited. Therefore, the aim of this proposal is to investigate the effects of naturally occurring polyphenolic antioxidants on obesity-related inflammation, adipocytokines and oxidative stress. In vitro and in vivo models evaluation of the effects of naturally occurring polyphenolic antioxidants on obesity-related inflammation, adipocytokines and oxidative stress and their molecular mechanisms. The purpose of this three year’s project is to investigate this problem as described above. In the first year’s plan, in vitro model evaluation of the effects of naturally occurring polyphenolic antioxidants (such as phenolic acids and flavonoids) on TNF-α induced inflammation, adipocytokines and oxidative stress in 3T3-L1 adipocyte. In the second year’s plan, in vivo models of evaluation of the naturally occurring polyphenolic antioxidants on inflammation, adipocytokines and oxidative stress in rats fed a high-fat diet. Further, the molecular mechanisms of naturally occurring polyphenolic antioxidants on inflammation, adipocytokines and oxidative stress in 3T3-L1 adipocytes and rats will be investigated in the third year’s plan. The overall plan expects to realize whether naturally occurring polyphenolic antioxidants reduce obesity-related inflammation and modulates that molecular mechanisms.肥胖是世界公共衛生與預防醫學上的重要課題。肥胖與慢性、低程度發炎反應之 關係密切，在脂肪細胞中會有不正常之細胞激素產生，以及活化發炎之訊息路徑。近 年來有研究指出，抗氧化物具有抑制肥胖相關發炎反應，其中N-乙醯半胱胺酸與白藜 蘆醇即被證實具有減低腫瘤壞死因子 (TNF-α)誘導之脂肪細胞激素分泌改變，但其它 之天然抗氧化物則鮮少有完整資料可詢，因此本研究計畫將針對天然多酚抗氧化物對 肥胖相關發炎反應、脂肪細胞激素與氧化壓力之影響進行探討。研究計畫以體外與體 內實驗模式評估天然多酚抗氧化物對肥胖相關發炎反應、脂肪細胞激素與氧化壓力之 影響及其分子機轉之探討，計畫內容擬分三年進行，第一年以體外實驗模式，探討天 然多酚抗氧化物 (酚酸化合物與類黃酮)對TNF-α誘導3T3-L1 脂肪細胞之發炎反應、 脂肪細胞激素與氧化壓力影響；第二年則以體內實驗模式，評估天然多酚抗氧化物 (由 第一年篩選)對以高脂飲食所誘導大鼠之發炎反應、脂肪細胞激素與氧化壓力影響；第 三年利用3T3-L1 脂肪細胞與動物試驗模式，探討天然多酚抗氧化物 (由第一年與第二 年篩選)對肥胖相關發炎反應、脂肪細胞激素與氧化壓力之分子機轉探討。此三年研究 計畫期望能了解天然多酚抗氧化物減低肥胖相關發炎反應，及其分子機轉調控。Project