2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38787Ochratoxin A and microcystin are two natural carcinogenic toxins. They are commonly contaminated in food, feed and water which cause toxic effects and cancer in human and animal. Production of antibodies and development of enzyme-linked immunosorbent assay (ELISA) for ochratoxin A and microcystin are important issue in food safety. However, ELISA method is usually multi-step and very difficult for automation. This drawback can be overcome by a new automated immunoassay method based on a fluorescence polarization immunoassay (FPIA). FPIA is based on the increase in polarization of the fluorescence of small fluorescent-labeled toxin (tracer) binding with specific antibody. Therefore, the first year objective of the present proposal is to to establish fluorescence polarization immunoassay (FPIA) for ochratoxin A and microcystin and apply this technology for rapid monitoring food, feed and water contamination. Recently, the application of molecularly imprinted polymers (MIP) in toxin detecting is promising. This MIP technology for identifying a particular molecule is attractive because of the simplicity of the preparation of the polymer and the simplicity and specificity of the identification of a target molecule. The advantage of this MIP technology is cheap and it can be reuse several times. Therefore, the second year objective of this proposal is focused on development of molecular imprinting technique for ochratoxin A and microcystin and apply this technology for rapid monitoring food, feed and water contamination.赭麴毒素A(ochratoxin A)與微囊藻毒(microcystin)是自然界兩種常見到的毒素，泛存於食品、作物與飲水中，食用遭受污染的食品會導致人類許多疾病及癌症的生成。由於此類毒素對於人類及動物體健康有高度的威脅，因此開發各式抗體及建立快速酵素免疫檢測分析法來分析調查各類食品中赭麴毒素與微囊藻毒的污染是一重要課題。但是酵素免疫檢測分析法步驟較多，難以自動化。此一缺點可由新穎的螢光極化免疫分析法(fluorescence polarization immunoassay, FPIA)來加以克服。 因此本計劃的第一年將建立赭麴毒素A與微囊藻毒之螢光極化免疫分析法，並且利用此一快速檢測技術來檢測分析食品作物中與飲水中此二類毒素污染情形。近年來由於分子拓印技術(molecularly imprinted polymers, MIP)的進展，利用分子拓印技術於毒素檢測及分析上的應用逐漸增加，分子拓印技術具有可簡單合成聚合物與可重複使用等優點，因此本計畫第二年將進行赭麴毒素A與微囊藻毒分子拓印技術開發及應用，將毒素當作模板，以此模板進行單體合成高分子聚合物，此高分子聚合物可取代傳統的抗體應用到酵素免疫分析法來檢測分析食品作物中與飲水中此二類毒素污染情形。赭麴毒素微囊藻毒螢光極化免疫分析法酵素免疫分析法分子拓印技術ochratoxin Amicrocystinenzyme-linked immunosorbent assay (ELISA)fluorescence polarization immunoassay (FPIA)molecularly imprinted polymers (MIP)Project