2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38579The diagnosis and treatment of male infertility rely on the conventional semen analysis (SA), which emphasizes the importance of sperm count, motility, and sperm morphology. However, the results of SA usually are unable to identify the underlying etiology of male infertility. In addition, unexpected fertilization failure occur when the results of conventional semen analysis are utilized to direct the treatment modality, such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). As a result, the research community suggests the application of advance semen analysis, including reactive oxygen specifies (ROS), apoptosis, and chromatin damage, for certain infertile couples. In this proposal, we will evaluate the effect of ROS on sperm capacitation and polypronucleate zygote formation in order to determinate the necessity to measure ROS levels in semen analysis. Second, we will evaluate the efficiency of removing apoptotic sperm and the effect on sperm capacitation by magnetic cell separation technique for patients with unexplained infertility and low fertilization rate during IVF treatment. Third, we will evaluate the efficiency of sperm selection for chromatin integrity by zona-binding and hyaluronic acid-bind ability for patients with oligospermia. The effect of zona pellucida and hyaluronic acid on sperm capacitation (acrosome reaction) will also be explored.男性不孕症的診斷與治療大部分依賴傳統的精液分析，但其結果往往無法診斷出病 因；用來指引體外受精的選項時，也偶有意外受精失敗的案例，故而學者呼籲對於 不孕症患者的診斷與治療上，進階的精液分析應當包括氧化壓力、細胞凋亡、及染 色質損壞的檢驗，而且進一步認為應當發展新的精液製備方式，去除掉細胞凋亡或 染色質異常之精子，以供體外受精或精蟲顯微注射使用。本計畫將先探討氧化壓力 對於體外受精的精子活化及受精率的影響程度，評估氧化壓力加入精液分析項目的 必要性。其次則是針對原因不明不孕症及體外受精率低的患者，評估以磁珠流式細 胞儀清洗並去除細胞凋亡精子的製備方式之效率，同時探討對於精子活化能力的影 響。然後是對於寡精症患者，評估使用玻尿酸或透明層結合篩檢精子的製備方式之 效率，同時探討頂體反應的發生狀況。藉此研究計畫瞭解進階的精液分析於臨床上 的必要性與應用價值，評估新進的精蟲選擇方式是否能真的有效去除細胞凋亡及染 色質異常之精子，以期能幫助婦女懷孕，並提升胎兒及寶寶的健康。精子活化氧化壓力細胞凋亡染色質體外受精精蟲顯微注射玻尿酸sperm capacitationreactive oxygen speciesapoptosischromatinin vitrofertilizationhyaluronic acidProject