LI-SUNG HSU2026-10-162026-10-161995-01-01Pure ID: 15286070Pure UUID: 36cf7de8-beab-4127-81e4-9ce9514ac44910.1042/bj3090445https://www.scopus.com/pages/publications/0028980280https://scholars.csmu.edu.tw/handle/123456789/28505To determine whether NG108-15 cells contain a functional Na+/Ca2+ exchanger, we isotonically replaced extracellular Na+ with N-methyl-D-glucamine (NMG) and measured the effect on cytosolic Ca2+ concentration ([Ca2+](i)) using the fluorescent Ca2+ indicator fura 2. Replacement with NMG alone had no effect on basal [Ca2+](i) or the rise in [Ca2+](i) evoked by 80 mM K+ or 10 μM bradykinin, but caused a larger [Ca2+](i) increase when thapsigargin and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) were added to the cells; this enhanced [Ca2+](i) increase could be reversed by adding Na+ back to the bathing buffer. The elevation in [Ca2+](i) induced by thapsigargin and FCCP was inversely proportional to extracellular Na+ concentration. Furthermore, the exchanger operated in the reverse mode, as measured by either [Ca2+](i) change or 45Ca2+ uptake. An 810 bp cDNA fragment of the exchanger was amplified by PCR; it differed by a single amino acid residue from the corresponding segment of the rat brain Na+/Ca2+ exchanger. These data suggest that a functioning Na+/Ca2+ exchanger exists in NG108-15 cells.8enjournal article