2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38680Particulate pollutant that interference the air visibility is also concerned associating with human respiratory health effect. For this reason, bioaerosol has also become an important issue, as Taiwan is located in a subtropical area. The warm weather and high moisture climate are extremely suitable in growing bioaerosols. In order to prevent workers working in biotechnology manufacturing industry aspirate bacteria and allergens, the employers should provide them necessarily personal protection equipments, PPE. The respiratory protection filters will damage human beings and environments once loading of airborne fungi and allergens in highly temperature and humidity atmosphere. For instance, the filtration quality of personal respiratory protection filters will reduce as continuing contacting with human sweats, skin, and other secrets. Moreover, the fungi and molds will breed on the filter media under high temperature and humidity condition, especially when the filter media are frequently used and are not disposable. The hyphae can possibly penetrate the filter media and develop into biohazard. In this study, the personal respirator filters, surgical masks, cotton masks, and paper masks will be tested for the reproductive of bacteria, and re-entrainment of bacteria. The operation parameters include the variability of temperature and humidity in growing bacteria, and the varying of filter reproductive of bacteria under different degree of storage condition. A Collison nebulizer will be used to generate challenge aerosol particles. A 20-mCi radioactive source, Am-241, will be used to neutralize the challenge particles to the Boltzmann charge equilibrium. Distilled water, artificial saliva and artificial perspiration will be used as the nutrient in growing bacteria. A single stage Andersen sampler will be used to sample bacteria for colony counting subsequently. The personal protective cloth and glove will be test for the protection efficiency. A Scanning Mobility Particle Sizer, SMPS, will be used to measure particle size upstream and downstream of the filter. The filter quality will be recounted and determined by filter reuse efficiency. The whole project can be further divided into five sequential sub-projects as follow: Year 1: Reuse Evaluation of Disinfection of Respirator Filters Year 2: Evaluation of Bacteria Reentrainment from Respirator Filters Year 3: Evaluation of Filters Moisture Regain and Bacteria Surviving Year 4: Performance Evaluation of Protective Cloth Year 5: Performance Evaluation of Protective Glove粒狀污染物除了造成視覺的干擾之外，最為關心的是會引起與呼吸道有關的 健康效應。近年來生物氣膠與呼吸道相關症狀被認為具高度相關性，而台灣整年 高溫高濕，相當適合生物氣膠微粒的生長。在以往生物性感染案例中，落實就業、 實驗場所的衛生管理，特別是要避免或降低生物性物質的暴露，例如：對於部分 接觸生物病原菌或過敏原之員工，應提供適當之個人呼吸防護具，使用加裝 HEPA 過濾濾材的生物安全櫃，以及安置過濾器於通風系統中，以避免工作人員 因吸入病菌與過敏原而導致疾病。 呼吸防護濾材在使用的過程中，若負載的對象為黴菌、真菌類生物性氣膠 時，極有可能在高濕度、高溫環境下造成濾材污染，並對人體、環境形成傷害。 例如：個人呼吸防護具或HEPA 過濾器內的濾材，若未定期更換，在重複使用下， 不斷與人體呼出之溫、濕氣體、排出之汗液或其他體液、皮屑之接觸，加上在局 部高溫、高濕度環境下，容易促使黴菌、真菌等於濾材上滋生繁殖，濾材的過濾 效果將大幅降低，而這些菌類的菌絲更可能穿透濾材延生至濾材背面，並進而使 人員或實驗室進行感染性微生物研究時，受到直接、間接的接觸或吸入等不同程 度的生物氣膠污染。 因此本研究目的在比較在不同的溫、濕條件下，不同的使用條件時，因生物 性氣膠之負載，而造成濾材的過濾特性變化、菌類於人工唾液、汗液培養下之繁 殖率，細菌負載於濾材上之再進入現象探討，並提供在不同的操作條件時，濾材 的建議使用期限。而呼吸防護濾材再回潮率與細菌存活率之關係也是一重要評估 指標。另外，對市面上常見的一般符合國際與國內相關測試規範要求之N95 防 塵口罩、醫用外科手術口罩、棉紗口罩、紙製口罩經過再生處理過後，測試不同 消毒方法對口罩上細菌之除污效率、殺菌效果，主要為測試其是否可在不影響過 濾效能情況下達到有效殺菌效果，以供其性能改善或選用之參考，進而促進產品 升級與民眾健康。另外，不同防護衣及防護手套其性能檢測、產品標準與防護效 果，消毒除污的效率也必須加以評估。將使用ASTM Standard Method，並分析、 檢測細菌於市售生物防護衣之防護特性，包含細菌于于布料上之附著、穿透、及 殘留菌數、脫附情形，以期瞭解其性質與防護效果，進而達到有效的防護工作， 確保民眾與醫療人員的健康。濾材之測試主要以柯利森霧化器產生挑戰氣懸微 粒。並以Am-241 中和所產生之氣懸微粒，使其達到波茲曼電量平衡的狀態。在 氣懸微粒濃度的量測上，主要安德森一階採樣器進行。粒徑之量測則主要以微粒 電移動度掃瞄分徑器（Scanning Mobility Particle Sizer, SMPS）進行量測。 因此本研究預計將分五年執行： 第一年：呼吸防護濾材消毒再利用評估 第二年：呼吸防護濾材再進入現象評估 第三年：呼吸防護濾材再回潮率與細菌存活率評估 第四年：防護衣防護效能評估 第五年：防護手套防護效能評估健康效應生物氣膠個人防護具回潮率再進入health effectbioaerosolPPEmoisture regainreentrainmentProject