2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38831Tissue engineering has emerged as a promising approach without the limitations of donor shortage and of synthetic prostheses that are not able to replace all the functions of a damaged or lost organ or tissue. To accommodate cells and guide their growth and tissue regeneration in three dimensions, a highly porous artificial extracellular matrix or scaffold is required in tissue engineering. A synthetic material used as scaffold must be biocompatible and it should exhibit some structural and mechanical equivalence to bone. Bone is a composite material and consists mainly of an organic matrix (collagen) and a mineral phase (hydroxyapatite). Thus, the successful design of bone substitute materials should be an analogue of the bone structure. Silicon, an important trace element in an early stage of bone formation, increases directly with calcium at relatively low calcium concentrations and falls below the detection limit at compositions approaching hydroxyapatite. The soluble form of Si may stimulate collagen type I synthesis and osteoblastic differentiation. Strontium element can also increase the bone formation and bone mineral density. Thus, the use of hybrid composites comprised natural polymers such as gelatin and chitosan and Sr-containing calcium silicate ceramics, resembling the morphology and properties of natural bone, may be one way to solve the problem of ceramics’ brittleness not only without reducing mechanical properties, but also possessing high bioactivity and great bone bonding ability. On the other hand, a successful scaffold should balance mechanical function with biofactor (growth factor) delivery, providing a sequential transition in which the regenerated tissue performs function as the scaffold degrades. In this three-year project, we aim at fabricating an advanced biomimetic scaffold that combines natural polymers and Sr-containing calcium silicates (SrCaSi) for use in bone tissue engineering. The effect of design strategies on mechanical and biological performances of the hybrid scaffold will be investigated. In the first year objective will optimize SrCaSi ceramics with polymeric materials using the characterization methods and in vitro evaluation in physiological solution. The processing parameters include precursor ratio, sintering temperature of powder, and polymer amounts. Next, the proliferation and osteogenetic expression of human osteosarcoma cell line MG63 for the novel materials will be evaluated. Gelatin (or chitosan) carriers for protein release will be also designed. Finally, the porous scaffold with mechanical properties comparable to the spongy bone will be constructed. The design and analysis of bilayer-structured (porous and dense) scaffolds with controlled protein release will be achieved. Combination of basic science and medical practice, results of this three-year research are expected to have a significant potency in the areas of biomaterials science and biotechnology.組織工程的目的是為克服因捐贈者短缺的移植難題，及突破人工材料不能夠取代所有受傷或缺損的器官或組織。為容納細胞且使其成長及組織再生，組織工程需要有三維多孔的細胞外基質或支架。人工合成支架材料必須具備生物相容性，且與骨組織相近的結構及機械性。骨組織是一種複合材，主要是由有機相（膠原蛋白）與無機相（HA）構成。是以成功的取代材設計應以骨組織本質為藍圖。 矽是早期骨形成階段的重要微量元素，在低鈣濃度時其含量隨鈣而增加，但當新生骨組成接近氫氧基磷灰石時，則含量低於偵測極限值。材料中溶解的矽可刺激第一型膠原蛋白合成及造骨細胞分化。鍶可以促進骨形成及增加骨密度。由天然高分子（明膠與幾丁聚醣）與鍶鈣矽酸陶瓷組裝成類似於自然骨組織形態與性質的複合材，不僅克服陶瓷脆性而不損其機械性質，且具有高生物活性及骨鍵結能力。另一方面，成功的組織工程支架應在生物力學功能與生物因子（生長因子）輸送上達成平衡，以便支架降解時，能提供後續傳輸以利再生組織進行生物功能。 本三年計劃的主要目標是結合天然高分子及矽酸鈣鍶，製造骨組織工程用之新式仿生支架，研究支架設計對仿生複合材機械及生物性能之影響。第一年計劃將經由各種材料基本分析與體外模擬測試，達成仿生複合材製程參數最佳化，製程參數包括溶膠-凝膠法的前導物比例、粉體燒結溫度及高分子含量等。第二年將利用MG63細胞株研究此具潛力的材料之刺激骨細胞增殖、分化及其基因表現。作為蛋白質釋放之明膠高分子（或幾丁聚醣）載體將一併於本年度製備。最後一年將構築機械性質與海綿骨相近之多孔支架，藉此並開發出同時擁有多孔及緻密之雙層結構特性的骨組織支架，且具蛋白質生長因子釋放功能。本三年計畫兼顧基礎研究及醫學應用，預期此三年研究結果在生醫材料及生物科技領域上會有其顯著效能。矽鈣化合物仿生複合材支架組織工程骨再生溶膠凝膠法Calcium silicateBiomimetic compositeScaffoldTissue engineeringBone regenerationSol-gelProject