Hui-Hsien PanJiunn-Liang KoChia-Ta WuHai-Lun SunYeak-Wun QuekMin-Sho KuKo-Huang Lue2026-01-302026-01-30202110.1159/000517184https://scholars.csmu.edu.tw/handle/123456789/4283<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Asthma animal models provide valuable information about the pathogenesis and the treatment of asthma. An ovalbumin (OVA)/complete Freund’s adjuvant (CFA)-sensitized model was developed to induce neutrophil-dominant asthma and to investigate whether fungal immunomodulatory peptide-&lt;i&gt;fve&lt;/i&gt; (FIP-&lt;i&gt;fve&lt;/i&gt;) could improve asthma features in the OVA/CFA-sensitized model. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; We used female BALB/c mice and sensitized them intraperitoneally with OVA/CFA on days 1, 2, and 3. On days 14, 17, 21, 24, and 27, they were challenged with intranasal OVA. The airway hyper-responsiveness (AHR) was detected by BUXCO, inflammatory cells were stained with Liu’s stain, the cytokines were detected using ELISA, and the airway inflammation was analyzed with hematoxylin and eosin stain. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; According to the results, OVA/CFA sensitization could induce AHR, high levels of IgE, and inflammatory cells especially neutrophils infiltration in the lung and airway inflammation. IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, IL-17, IL-25, IL-33, and transforming growth factor-β (TGF-β) increased in the OVA/CFA-sensitized mice. OVA/CFA-sensitized mice treated with FIP-&lt;i&gt;fve&lt;/i&gt; not only increased IL-12 and IFN-γ but also decreased IL-4, IL-5, IL-6, IL-8, IL-13, IL-17, IL-25, IL-33, and TGF-β in the bronchoalveolar lavage fluid. Moreover, FIP-&lt;i&gt;fve&lt;/i&gt; significantly decreased neutrophil infiltration in the lung. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; The OVA/CFA model induced neutrophilic asthma successfully, and FIP-&lt;i&gt;fve&lt;/i&gt; improved neutrophil-dominant asthma. </jats:p>[SDGs]SDG5journal-article