2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37948An ideal root end filling material should be biocompatible with dental tissues and create a local environment that is conductive to pulpal healing or osseous healing. The root end filling materials has been studied in our laboratory. The previous studies are found that the root end filling materials treated U2OS cells are dose dependent biocompatibility. At high concentrations of the root end filling material showed that U2OS cells apoptotic change. The other findings showed that mineral trioxide aggregate is cytocompatible with U2OS cell line. The DNA fragment assay of the U2OS cells, after treated with MTA, showed that no DNA fragmentation. The MAPK kinase regulation of the MTA treated cell is by ERK kinase pathway. These results showed clinically used root end filling materials are biocompatible in vitro. But there is scant article discussed root end filling material treated on animal in vivo. The purpose of the present study is to compare the effects of different root end materials implanted in Balb/c mice. The cytokine expression, pathological change on tissue and biochemical assay of the blood are planning to explore. It is hoped that present study can offer more information on the selecting root end filling materials.Root end filling materials cytokines in vivo testProject